Technical Data Sheet
Size Exclusion Chromatography Columns ROTI®Dex-25 Superfine FPLC 1 ml, 100 unit(s)
Sterile products
Ready-to-use products and reagents
Flash point (flp) 43 °C
Storage temp. +2 to +25 °C
WGK 2
Suitable for proteins >5 kDa and oligonucleotides >10 bp.
€7,519.65/Pack Qty.
excl. VAT. | 100 unit(s) per Pack Qty.
Art. No. 21C6.2
Alternative products
Product details
Prepacked ROTI®Dex-FPLC columns for size exclusion chromatography using syringe, pump or FPLC systems, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.
- 'Fast Protein Liquid Chromatography' (FPLC)
- Common chromatographs such as ÄKTA™FPLC™
- Syringe and peristaltic pumping systems
The purification is based on a spherical porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.
In size exclusion chromatography molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.
In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.
- Sample preparation in just a few minutes
- Desalting, removal of impurities and buffer exchange in a single step
- No interaction between analyte and medium
- High selectivity, high resolution and high yield (> 95%)
- Also suitable for small sample volumes
- High chemical stability and therefore compatible with many organic and other solvents
Size Exclusion Chromatography Columns ROTI®Dex-25 Superfine FPLC 1 ml
The column bed consists of ROTI®Dex-25 Superfine. It is the gel filtration medium with the smallest dextran particles, which provide efficient results and very high resolutions. Biomolecules >5 kDa or >10 bp and particles >2 nm are purified. The fractionation range for proteins/peptides/biomolecules is between 1 and 5 kDa, with the highest resolution (most efficient separation) achieved between biomolecules >5 kDa and <1 kDa.
- Oligonucleotides > 10 bp
- Globular proteins > 5 kDa
- Particles > 2 nm
Sample volumes of 0.05 - 0.3 ml are recommended for the 1 ml columns.
Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.
| Version | 1 ml bed volume |
| Matrix | Cross-linked dextran |
| MWCO |
5 kDa 10 bp |
| Particle size |
Dry: 20-50 µm Wet:40-110 µm |
| Storage buffer | 20 % (v/v) ethanol |
| Sample volume | 0.05 – 0.3 ml |
| Max. pressure | 3 bar (0.3 MPa) |
| recommended flow rate | 0.5 - 2 ml/min |
| maximum flow rate | 3 ml/min |
| Column shape | 0.7 cm inner diameter x 2.5 cm height |
| Inlet connection | 10–32 (1/16”) female |
| Outlet connection | 10–32 (1/16”) male |
- Subtotal: 0.00
| Art. No. | Version | Pack Qty. | Price | Quantity | |
|---|---|---|---|---|---|
| 21C6.1 | 1 ml bed volume | 5 unit(s) |
€428.95 |
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| 21C6.2 | 1 ml bed volume | 100 unit(s) |
€7,519.65 |
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In stock
Available
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
The ROTI®Dex ready-to-use columns at a glance
| Application | Order No. | Matrix | Particle size (wet) | Gel bed volume | Sample volume | MWCO | Pack Qty. |
| Spin | 21C4.1 | ROTI®Dex 25 Medium | 85-260 µm | 0.5 ml | 2-100 µl (50 µl optimal) | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
25 Pcs |
| 21C5.1 | ROTI®Dex 50 Medium | 100-300 µm | 0.5 ml | 2-100 µl (50 µl optimal) | Proteins >25 kDa, Oligonucleotides >20 bp, Nanoparticles >4 nm |
25 Pcs | |
| Grav | 21AX.1 | ROTI®Dex 25 Medium | 85-260 µm | 1.78 ml | 0.15-0.3 ml | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
50 Pcs |
| 21AY.1 | 2.75 ml | 0.5 ml | 50 Pcs | ||||
| 21C0.1 | 4.31 ml | 1 ml | 50 Pcs | ||||
| 21C1.1 | 10.37 ml | 2.5 ml | 25 Pcs | ||||
| 21C2.1 | 17.2 ml | 5 ml | 10 Pcs | ||||
| 21C3.1 | 34.21 ml | 10 ml | 10 Pcs | ||||
| FPLC | 21C6.1 | ROTI®Dex 25 Superfine | 40-110 μm | 1 ml | 0.05-0.3 ml | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
5 Pcs |
| 21C6.2 | 1 ml | 0.05-0.3 ml | 100 Pcs | ||||
| 21C7.1 | 5 ml | 0.1-1.5 ml | 5 Pcs | ||||
| 21C7.2 | 5 ml | 0.1-1.5 ml | 25 Pcs | ||||
| 21C7.3 | 5 ml | 0.1-1.5 ml | 100 Pcs (4x25) |
Technical data of the ROTI®Dex FPLC columns at a glance:
| Order No. | 21C6.1 | 21C6.2 | 21C7.1 | 21C7.2 | 21C7.3 |
| Package size | 5 Pcs | 100 Pcs | 5 Pcs | 25 Pcs | 100 Pcs |
| Matrix | ROTI®Dex 25 Superfine | ||||
| Particle size (wet) | 40-110 μm | ||||
| Gel bed volume | 1 ml | 5 ml | |||
| Sample volume | 0.05-0.3 ml | 0.1-1.5 ml | |||
| Pressure max. | 3 bar (0.3 MPa) | ||||
| Flow rate max. | 3 ml/min | 10 ml/min | |||
| Recommended flow rate | 0.5-2 ml/min | 1-5 ml/min | |||
| Outlet port | 10-32 (1/16″) male | ||||
| Inlet port | 10-32 (1/16″) female | ||||
| Column size | 0.7 cm inner diameter x 2.5 cm height | 1.6 cm inner diameter x 2.5 cm height | |||
Certificates of Analysis
Type analysis
| Packing evaluation Asymmetry: (5 % CV 2M NaCl salt plug; flow rate 1 mL/min, run in PBS) | 0.7 ≤ As ≤ 1.6 |
| Overlap of peak areas: (20 % CV (0.2 mL) 2 g/L BSA and 2 M NaCl; flow rate 1 mL/min, run in PBS) | ≤10 % |