ROTI®Garose-Protein A/G HPBeads, 1 ml
Flash point (flp) 35 °C
Melting point (mp) -114 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170
Suitable for LPLC, FPLC and MPLC and big matrix volumes
In ROTI®Garose-Protein A/G HPBeads, the advantages of the highly efficient protein A and G/antibody binding have been combined with a bead technology allowing a very rapid flow rate.
For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases. ROTI®Garose-Protein A/G HPBeads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
€187.50/Pack Qty.
excl. VAT. | 1 ml per Pack Qty.
Art. No. 0809.2
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Product details
ROTI®Garose-Protein A/G HPBeads ROTI®Garose for biochemistry
Mixture (1:1) of protein A- and protein G coated agarose beads for affinity chromatography under medium pressure, with high flow rate or with big sample-/matrix volume. High-performance affinity resin for antibody purification. 50 % bead suspension in 20 % ethanol.
In ROTI®Garose-Protein A/G HPBeads, the advantages of the highly efficient protein A and G/antibody binding have been combined with a bead technology allowing a very rapid flow rate.
For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases. ROTI®Garose-Protein A/G HPBeads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
- Rapid one-step purification of immunoglobulins from cell lysates and biological solutions
- High binding capacity for all IgG populations in mammals
- High yield of very pure immunoglobulins
- Easy elution and regeneration
- Covalent binding of protein G in high ligand density
- For batch mode, gravity flow, MPLC und FPLC
- Recommended for big matrix volumes
All small-scale applications, for MPLC and FPLC, or if antibodies shall be isolated either in short time or in big amounts.
The matrix of ROTI®Garose-Protein A/G HPBeads consists of crosslinked and beaded 4 % agarose, coated and covalently coupled with protein A from Staphylococcus aureus and protein G from Streptococcus, respectively. recprotein A shares IgG binding properties with natural protein A of S. aureus Cowan strain I. Binding domains specific for albumin as well as cell walls and cell membranes have been removed in the recombinant protein G, in order to ensure the maximum specific IgG binding capacity.
The recombinant protein A has high affinity for IgG from a variety of different mammalian species, also binding some populations of IgA and IgM. The recombinant protein G has high affinity for IgG from a variety of different mammalian species, also binding IgG3 with high affinity.
Most immunoglobulins may be eluted in 100 mM glycin or citric acid buffer (pH 3.0).
ROTI®Garose-Protein A/G HPBeads may be regenerated, making them very cost-effective. May not be autoclaved.
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| Order No. | Pack Qty. | Pack. | Price | Quantity | ||
|---|---|---|---|---|---|---|
| 0809.1 | 0.5 ml | plastic | €114.50 |
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| 0809.2 | 1 ml | plastic | €187.50 |
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| 0809.3 | 2 ml | plastic | €332.50 |
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Downloads
Certificates of Analysis
Type analysis
| Particle size | |
| Beads (cross-linked agarose) | 4 % |
| Ligand | recombinant protein A/G |
| Chemical bonding | covalent |
| Binding capacity (humane IgG) | ~25 mg/ml |
| Ethanol | 20 % |