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Albumin, IgG free, US-Origin, 10 g

≥98 %, IgG-free, for biochemistry and molecular biology
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Pack.
BSA
Molar mass (M) ~66 000 g/mol
Storage temp. +4 °C
WGK 1
CAS No. [9048-46-8]
EG-Nr. 232-936-2

Recommended as blocking and stabilising reagent in all antibody-mediated detection systems.
Heatshock albumin.
Recommended if BSA with US-Origin is required.

Albumin, highly purified and tested for the presence of immunoglobulins (IgGs)
The Albumin, IgG-free, US-Origin is especially recommended to stabilize antibody solutions and as blocking reagent in all assays working with antibodies. Due to its particularly low protease content, it is also well suited for use in all other protein assays that could be interfered with by protease presence.
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Guarantee analysis

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Art. No. 1ET9.1

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Product details



Albumin, IgG free, US-Origin ≥98 %, IgG-free, for biochemistry and molecular biology

Albumin, highly purified and tested for the presence of immunoglobulins (IgGs)
The Albumin, IgG-free, US-Origin is especially recommended to stabilize antibody solutions and as blocking reagent in all assays working with antibodies. Due to its particularly low protease content, it is also well suited for use in all other protein assays that could be interfered with by protease presence.


For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system.


Tested for the absence of detectable IgG.



Albumin, IgG free, US-Origin
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1ET9.1 10 g plastic €59.90
1ET9.2 50 g plastic €158.90
1ET9.3 200 g plastic €465.50
1ET9.4 500 g plastic €939.00
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General information

Recommended Application of Albumins

Albumin Art. No. Recommended Application
Fraction V, Europe 1ETA Stabilisation of proteins and blocking reagent in protein biochemical detection systems. Basically suitable for all assays unless specific requirements apply.
Fraction V, US-Origin 3854 Stabilisation of proteins and blocking reagent in all standard biochemical detection systems. Basically suitable for all assays unless specific requirements apply. Recommended if albumin with certified origin USA has to be used.
Fraction V, NZ-Origin 8076 Stabilisation of proteins and blocking reagent in protein biochemical detection systems. Basically suitable for all assays unless specific requirements apply. Recommended if albumin with certified origin New Zealand or Australia has to be used.
Fraction V, pH 5.2, Europe 2834 Stabilisation of proteins and blocking reagent. Particularly recommended for Western-Blotting and ELISAs.
pH 5.2, US-Origin (heat shock) 1ET8 Stabilisation of proteins and blocking reagent. Particularly recommended for Western-Blotting and ELISAs. Recommended if albumin with certified origin USA has to be used.
sufhydryl-blocked, NZ-Origin (heat shock) 1ET3 Particularly sensitive assays where monomeric albumin is guaranteed to be used or where reproducibility is particularly important. Can be stored as monomers over a longer period of time.
Fraction V, biotin-free, NZ-Origin 0163 Stabilisation of proteins and blocking reagent in all biotin / streptavidin-mediated detection systems (membrane-bound- or coating-plate-assays, as well as soluble assays).
Fraction V, protease-free, Europe T844 Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays.
protease-free, US-Origin (heat shock) 1ET5 Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays. Recommended if albumin with certified origin USA has to be used.
protease-free, NZ-Origin (heat shock) 1ET7 Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays. Recommended if albumin with certified origin New Zealand or Australia has to be used.
IgG-free, US-Origin (heat shock) 1ET9 Stabilisation of antibodies. Blocking reagent in all antibody-mediated detection systems and Radio-Immuno Assays (RIAs). Reduces false-positive signals. Recommended if albumin with certified origin USA has to be used.
IgG-free, NZ-Origin (heat shock) 3737 Stabilisation of antibodies. Blocking reagent in all antibody-mediated detection systems and Radio-Immuno Assays (RIAs). Reduces false-positive signals. Recommended if albumin with certified origin New Zealand or Australia has to be used.
Fraction V, fatty acid-free, Europe 0052 Stabilisation of proteins and blocking reagent in detection systems. Particularly recommended for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins.
fatty acid-free, US-Origin, (heat shock) 9638 Particularly for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins. Also well suited for cell biology and all antibody-mediated detection systems. Recommended if albumin with certified origin USA has to be used.
fatty acid-free, NZ-Origin (heat shock) 1ETC Stabilisation of proteins and blocking reagent in detection systems. Particularly for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins. Recommended if albumin with certified origin New Zealand or Australia has to be used.
very low endotoxin, US-Origin (heat shock) 1ET6 Cell culture of all cell lines, primary cells, stem cells. Cloning and 3D-cultures or cell-stressing assays like transfections, in vitro infections, cell fusion. Recommended if albumin with certified origin USA has to be used.
very low endotoxin, NZ-Origin (heat shock) 1ET4 Cell culture of all cell lines, primary cells, stem cells. Cloning and 3D-cultures or cell-stressing assays like transfections, in vitro infections, cell fusion. Recommended if albumin with certified origin New Zealand or Australia has to be used..

Molar extinction coefficient: 4,4x104 L x mol-1 x cm-1 (acc. to 0,667 L x mol-1 x cm-1 for a 0,1 % solution).


BSA are proteins yielded from blood, which function as binding and transport proteins in blood circulation. Depending on the preparation process, the albumins contain different quality and quantities of metabolic products, enzymes, peptides, fatty acids, vitamines, etc. and are suitable for different applications.


Certificates of Analysis

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The following analysis certificates have been found:

Guarantee analysis

Appearancepowder to flakes
Colourpale yellow to greenish
OriginUSA
Assay≥98 %
Protein≥98 %
Loss on drying≤5 %
Solution (4 % in H2O)complies
pH value (10 % in H2O)6.5-7.5
Sulphated ash≤2 %
Sodium (Na)tested
Chloride (Cl)tested
Calcium (Ca)tested
Potassium (K)tested
Phosphor (P)tested
Heavy metals≤10 ppm
IgG (COBAS)none detected
Protease≤0.005 U/mg

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