Technical Data Sheet
ROTI®Spin MINI, 25 unit(s), 5 kDa
€116.00/Pack Qty.
excl. VAT. | 25 unit(s) per Pack Qty.
Art. No. 3L59.1
Alternative products
Fits to / Accessories
Product details
For purification and concentration of biomolecules and for purification of solutions.
- ROTI®Spin MINI: 1.5 ml sample tube for up to 500 µl sample
- ROTI®Spin MIDI: 15 ml sample tube for up to 5 ml sample
- ROTI®Spin MAXI: 50 ml sample tube for up to 15 ml sample
Centrifugal filters for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates.
ROTI®Spin Centrifugal filters can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled.
Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC.
| Overview for selecting the appropriate pore size | ||
| MWCO | Molecule size | DNA size |
| 10 kDa | 30 – 90 kDa | 50 – 200 bp |
| 30 kDa | 90 – 180 kDa | 200 – 500 bp |
| 50 kDa | 150 – 300 kDa | 500 bp – 1 kb |
| 100 kDa | >300 kDa | >1 kb |
ROTI®Spin MINI
Sample volumes:
Initial volume: 50-500 μl
Final volume: 10-20 μl
MWCO:
Centrifugation units with membranes in 5 different molecular weight cut-offs (MWCO) are available for specific applications. Select the appropriate MWCO according to the following rule of thumb:
Purification of proteins/peptides: MWCO ≤1/3 of the sample molecule size.
Purification of nucleic acids: MWCO ≤1/2 of the sample molecule size.
- Suitable for nucleic acids, proteins/peptides, other biomolecules >200 kDa, and very small bioparticles
- Replaces standard applications such as precipitation, dialysis, gel filtration, gel purification, column chromatography, gradient centrifugation, etc.
- Gentle: no shearing of DNA up to 100 kb, proteins remain native and enzymatically active
- Can be used without restriction on labeled samples (isotope, fluorescence, chromogenic labeling)
- Very high recovery rates: with a suitable MWCO, the yield is >90%.
| MWCO | 5 kDa |
- Subtotal: 0.00
| Art. No. | Pack Qty. | MWCO | Price | Quantity | |
|---|---|---|---|---|---|
| 3L59.1 | 25 unit(s) | 5 kDa |
€116.00 |
|
|
| 3L59.2 | 96 unit(s) | 5 kDa |
€371.00 |
|
|
| 3L59.3 | 500 unit(s) | 5 kDa |
€1,700.00 |
|
|
| 3L5A.1 | 25 unit(s) | 10 kDa |
€116.00 |
|
|
| 3L5A.2 | 96 unit(s) | 10 kDa |
€371.00 |
|
|
| 3L5A.3 | 500 unit(s) | 10 kDa |
€1,700.00 |
|
|
| 3L5C.1 | 25 unit(s) | 30 kDa |
€116.00 |
|
|
| 3L5C.2 | 96 unit(s) | 30 kDa |
€371.00 |
|
|
| 3L5C.3 | 500 unit(s) | 30 kDa |
€1,700.00 |
|
|
| 3L5E.1 | 25 unit(s) | 50 kDa |
€116.00 |
|
|
| 3L5E.2 | 96 unit(s) | 50 kDa |
€371.00 |
|
|
| 3L5E.3 | 500 unit(s) | 50 kDa |
€1,700.00 |
|
|
| 3L5H.1 | 25 unit(s) | 100 kDa |
€116.00 |
|
|
| 3L5H.2 | 96 unit(s) | 100 kDa |
€371.00 |
|
|
| 3L5H.3 | 500 unit(s) | 100 kDa |
€1,700.00 |
|
|
|
In stock
Available (note delivery time)
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00