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Technical Data Sheet
1NNP 1NN7 1NNP_offen

anti-HA-tag mouse monoclonal, 12CA5, 25 µg
From  PROGEN
lyophilized, monoclonal, affinity purified
Icon_Abweichende-Konditionen Bitte beachten: Abweichende Konditionen / Please note: Different conditions
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Pack Qty.
Pack.
Primary antibodies
Density (D) ~1 g/cm³
Boiling point (bp) 100 °C
Storage temp. +4 °C
Transport temp. ambient temp.

0.25 mg/ml after reconstitution with 100 μl / 400 μl PBS
Binding of HA-tag proteins

Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
Product details

€112.50/Pack Qty. 

excl. VAT. | 25 µg per Pack Qty.

Art. No. 1NN7.1

Available (note delivery time)
Ready for dispatch in 1-2 weeks
Free of shipping costs from 250 €
from 6 Pack Qty. €106.88/Pack Qty.
from 24 Pack Qty. €101.25/Pack Qty.

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Product details


Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.

  • Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
  • High affinity to the respective protein tag
  • Robust & highly specific detection of the fusion protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-HA-tag mouse monoclonal, 12CA5 lyophilized, monoclonal, affinity purified

The monoclonal 12CA5 antibody recognizes the HA-tag (hemagglutinin tag). The HA-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.


Application

Western Blot (WB): 1:50,000-1:100,000 (0.005-0.0025 μg/ml)


1NN7
Western blot analysis of E. coli lysate containing HA-tagged protein with anti-HA-tag antibody. Western blot analysis was performed on 10 ng or 5 ng of E. coli lysate containing HA-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5 % dry milk in PBST for 1 h at RT. The primary antibody anti-HA-tag mouse monoclonal, 12CA5 (Cat. No. 910HAL) was diluted in blocking buffer (antibody concentration 0.005 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.


Not a medical device / Not an IVD product
Technical Information
Application WB 
Host species mouse 
Clonality monoclonal 
Purification Affinity chromatography 
Conjugate uncojugated 
Isotype IgG2b 
Clone 12CA5 
Target HA-Tag 
anti-HA-tag mouse monoclonal, 12CA5
Selected quantity:   0
  1. Subtotal:  0.00
Art. No. Pack Qty. Pack. Price Quantity
1NN7.1 25 µg plastic

€112.50

1NN7.2 100 µg plastic

€369.00

In stock
Available (note delivery time)
In procurement
No longer available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

Instruction / German

Specification / German

Instruction / English

Specification / English

Specification / French
Security datasheet
Germany / German
Security datasheet
International / English

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