Technical Data Sheet
anti-HA-tag mouse monoclonal, 12CA5, 100 µl
Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Boiling point (bp) 100 °C
Storage temp. +4 °C
Transport temp. cooled
€115.00/Pack Qty.
excl. VAT. | 100 µl per Pack Qty.
Art. No. 1NN7.1
Fits to / Accessories
Product details
Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
- Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
- High affinity to the respective protein tag
- Robust & highly specific detection of the fusion protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-HA-tag mouse monoclonal, 12CA5 250 µg/ml, monoclonal, affinity purified
The monoclonal 12CA5 antibody recognizes the HA-tag (hemagglutinin tag). The HA-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Western Blot (WB): 1:50,000-1:100,000 (0.005-0.0025 μg/ml)
| Application | WB |
| Host species | mouse |
| Clonality | monoclonal |
| Purification | Affinity chromatography |
| Conjugate | uncojugated |
| Isotype | IgG2b |
| Clone | 12CA5 |
| Target | HA-Tag |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 1NN7.1 | 100 µl | plastic |
€115.00 |
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| 1NN7.2 | 400 µl | plastic |
€375.00 |
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In stock
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- Subtotal: 0.00
Downloads / MSDS
General information
Secondary antibodies do not bind directly to the target molecule, but rather to the primary antibody. They are usually coupled with enzymes or fluorescent dyes so that the binding of the primary antibody can be made visible or measurable. In research, they serve as signal amplifiers and enable more sensitive detection, as several secondary antibodies can bind to one primary antibody. This makes them important tools in methods such as ELISA, Western blot or microscopy.
Primary antibodies are proteins that bind specifically to a particular antigen, i.e. a target molecule such as a protein or a structure within a cell. They are used in research to visualise specific molecules, isolate them or investigate their function. Typical applications include Western blotting, immunohistochemistry and flow cytometry. Due to their high specificity, primary antibodies enable the precise detection of biological processes.