X-Gluc, 100 mg
Molar mass (M) 539,81 g/mol
Melting point (mp) 230 °C
Storage temp. –20 °C
Transport temp. ambient temp.
CAS No. [114162-64-0]
excl. VAT. | 100 mg per Pack Qty.
Art. No. 0018.1
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X-Gluc ≥99 %, for microbiology
For colorimetric detection of glucuronidase activity. During hydrolysis of X-gluc two molecules are formed, glucuronic acid and 5-bromo-4-chloro-indoxyle, which is then being oxidized to a deep-blue indigo-dye.
X-Gluc is used for detection of contaminations by E. coli bacteria in food, waters and feeds. In molecular genetics, it is widely used as marker for expression analysis of target genes.
|Signal product||Precipitate (detection of living cells)|
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Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.
Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).
Always prepare freshly!
Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.
Reference: Bos, E.S. et al., (1981) J. Immunoassay 2, (3/4), 187.
|Appearance||white to off-white powder|
|Purity (HPLC)||≥99.0 %|
|Solution (1% in DMF/Wasser 1:1)||clear, colorless|
|Water (KF)||≤5.0 %|
|Specific rotation [α]a (c=1 in DMF/water 1:1)||-91.0° to -87.0°|