Synergel™, 100 g
Transport temp. ambient temp.
WGK 1
CAS No. [9000-40-2]
EG-Nr. 232-541-5
€225.25/Pack Qty.
excl. VAT. | 100 g per Pack Qty.
Art. No. 0184.1
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Product details
- Enhances separation of nucleic acid fragments
- For extremely clear gels
- Facilitates image documentation
- Optimal display especially with small fragments
Synergel™ ≥99 %, for gel electrophoresis, up to 30 kb
Synergel™ is a synergistic gelling and sieving agent consisting of a modified polysaccharide which, when combined with agarose, forms a hydrogen bonded binary gel system.
The addition of Synergel™ to agarose improves gel performance by providing superior separation and definition of DNA fragments up to 30 kb. A gel mixture containing 0,7 % agarose and 0,7 % Synergel™, e.g., will produce improved results when compared to a 2 % agarose-only gel.
Synergel™ also provides greater optical clarity, which allows higher quality photodocumentation of stained gels.
Standard buffer systems like phosphate, acetate or borate buffered Tris-EDTA may be used in forming the gel with agarose/ Synergel™. RNA may also be separated in this system by using a 2,2 M formaldehyde-containing buffer. Synergel™ is compatible with standard blotting procedures, thus, nucleic acid may be electroeluted or transferred to membranes following standard Southern/Northern blotting protocols.
The figure compares separation qualities of
A: 1,5 % Synergel™ / 0,7 % agarose and B: 4 % high-resolution agarose (NuSieve®).
Please see also figures for pUC19 markers T149 and X901.
| Application | Agarose additive for finer pore formation. Increases the separative power of the agarose. For fragments from 10 bp |
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| Order No. | Pack Qty. | Pack. | Price | Quantity | ||
|---|---|---|---|---|---|---|
| 0184.1 | 100 g | plastic | €225.25 |
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| 0184.2 | 10 g | plastic | €32.15 |
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Certificates of Analysis
Type analysis
| Colour | white to off-white powder |
| Odour | slightly salty |
| pH (1 % solution) | 9 |
| DNase, RNase | none detected |