Albumin, IgG free, 10 g
Storage temp. +4 °C
CAS No. [9048-46-8]
excl. VAT. | 10 g per Pack Qty.
Art. No. 3737.1
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Molar extinction coefficient: 4,4x104 L x mol-1 x cm-1 (acc. to 0,667 L x mol-1 x cm-1 for a 0,1 % solution).
Albumin, IgG free >98 %, IgG-free, for biochemistry and molecular biology
Albumin, highly purified and tested for presence of immunoglobulins (IgGs), proteases, RNAse, and DNAse. Albumin, IgG-free is particularly recommended for use as stabilising reagent in antibody solutions, and as blocking reagent in all assays using detection systems via antibodies.
Also very well suited for stabilising of hot-start-PCRs mediated by antibodies, or as blocking reagent in antibody-detected hybridisations.
For purification of Albumin, IgG-free, an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system.
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|Albumin||Art. No.||Recommended Application|
|Fraction V||8076||Stabilisation of proteins and blocking reagent in protein biochemical detection systems. Basically suitable for all assays unless specific requirements apply (see below-listed albumins).|
|Albumin solution 20 %||9400||Wide range of applications, e.g. as protein standard or blocking reagent.|
|Albumin solution 30 %||9401||Wide range of applications, e.g. as protein standard or blocking reagent.|
|Fraction V, US-Origin||3854||Stabilisation of proteins and blocking reagent in all standard biochemical detection systems. Recommended if albumin with certified origin USA has to be used.|
|Albumin solution 30 %, with azide, US-Origin||9414||Suitable as blocking or stabilising reagent in all general assays. Recommended if BSA with US-Origin is required.|
|Fraction V, pH 5.2||2834||Stabilisation of proteins and blocking reagent. Particularly recommended for Western-Blotting and ELISAs|
|Fraction V, protease-free||T844||Stabilisation of proteins and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays. Also recommended for stabilisation of antibodies.|
|Fraction V, biotin-free||0163||Stabilisation of proteins and blocking reagent in all biotin / streptavidin-mediated detection systems (membrane-bound- or coating-plate-assays, as well as soluble assays).|
|Fraction V, fatty acid-free||0052||Stabilisation of proteins and blocking reagent in detection systems. Particularly recommended for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins.|
|Albumin, fatty acid-free, US-Origin||9638||Stabilisation of proteins and blocking reagent, particularly for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins. Also well suited for cell biology and all antibody-mediated detection systems. Recommended if albumin with certified origin USA has to be used.|
|Albumin, lgG-free||3737||Stabilisation of antibodies. Blocking reagent in all antibody-mediated detection systems and Radio-Immuno Assays (RIAs). Reduces false-positive signals.|
|Fraction V, endotoxin-tested||CP84||Cell culture, standard cell lineages, cell sorting, short-term assays.|
|Fraction V, very low endotoxin||CP77||Cell culture. Superior quality and purity, suitable for sensitive cells like primary cells, stem cells, cloning and 3D-cultures. Also recommended for cell stressing assays like transfections, in vitro infections, or cell fusion.|
|Lactalbumin||7597||Cell culture, fermentation procedures, tissue culture.|
|Colour||yellow to beige|
|Assay (HPLC)||>98 %|
|Dry extract||≥94 %|
|Iron (Fe)||<15 mg/g|
|Sodium (Na)||<10 mg/g|
|Chloride (Cl)||<0,5 %|
|Solution (2 % in H2O)||soluble|
|pH value (2 % in H2O)||6,5-7,5|
|IgG (HPLC)||none detected|