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Rhodamine-12-dUTP, 50 µl, 50 nmol

≥95 %, 1 mM solution
Icon_DNAse-free
Icon_RNAse-free
Icon_Protease-free
Icon_PCR-inhibitor-free
Pack Qty.
Pack.
Packaging
Rhodamine-6-carboxyaminocaproyl-[5-(3-aminoallyl)-2'-desoxyuridine-5'-triphosphate]
Empirical formula C39H41N6O19P3
Molar mass (M) 990,7 g/mol
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. cooled
WGK 1

For non-radioactive, enzymatic labelling of DNA.

€322.00 /Pack Qty.

€225.35/Pack Qty.  Campaign price!

excl. VAT. | 50 µl per Pack Qty.

Art. No. 1049.2

Available at short notice
Free of shipping costs from 125 €
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Product details


  • DNAse-, RNAse-, Protease- and Phosphatase-free
  • Free of PCR inhibitors like modified bases and tetra-pyrophosphate
  • Adjusted to pH 7,5 for optimzed enzyme compatibility
  • Highly efficient enzymatic fabrication
  • Can be optimally combined with Carl ROTH ROTI®Pol DNA polymerases

Suitable for

PCR, cDNA synthesis, labelling and primer-extension, mutagenesis-assays, sequencing reactions and in vitro transcription.


All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also ’long range PCR’, repeated quantitative light-cycling reactions, and tests for physical stability.



Rhodamine-12-dUTP ≥95 %, 1 mM solution

Rhodamine-12-dUTP is able to replace dTTP in growing DNA-strands and is used for efficient non-radioactive DNA-labelling. Detection of labelled nucleic acids can easily be done by direct analysis of fluorescent signals (e.g. by fluorescence microscopy).
Rhodamine-12-dUTP can also be used for double staining techniques in combination with fluorescein-12-dUTP or biotin-11-dUTP and corresponding antibodies or streptavidin-complexes, respectively.

Excitation: 505 nm
Emission: 530 nm (red)


Application examples

Non-radioactive labelling of DNA by enzymatic reactions, e.g. PCR, reverse transcription, nick-end-translation, end-labelling or random-primed DNA-labelling. Incorporation can be done with all established DNA-polymerases (e.g. Taq-polymerase, T4 DNA-polymerase, Klenow fragment).
Tested for the lack of endo-, exodeoxyribonuclease, ribonuclease and phosphatase.


Rhodamine Green, mixture of 5/6 isomeres

ε505 (pH 7) = 8,5 E x mmol-1 x cm-1; pH: 7,5 ±0,2

Nukleotide_Stabilitaet
Figure 3:
Stability testing: HPLC analysis of all four dNTPs following incubation periodes of 1–14 days at different temperatures (4 °C – green; room temperature – red; 40 °C – blue). Stability of nucleotides is >99 % even after 14 days incubation at room temperature. Even after an incubation period of 9 days at 40 °C, 85 % of all nucleotide molecules are still intact.


Rhodamine-12-dUTP
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1049.1 25 µl plastic 25 nmol €132.05
1049.2 50 µl plastic 50 nmol €225.35
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