Proteinase K, 5 g, 1 x 5 g
WGK 1
CAS No. [39450-01-6]
EG-Nr. 254-457-8
Art. No. 7528.3: A The stock solution (20 mg/ml) can be prepared directly in the bottle.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
€1,627.80/Pack Qty. Campaign price!
excl. VAT. | 5 g per Pack Qty.
Art. No. 7528.6
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Product details
Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNase and DNase not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.
Stock solution: 20 mg/ml in water
Storage temperature (stock solution): -20 °C
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
Working solution: 50 µg/ml
Proteinase K ≥30 mAnson U/mg, lyophilized
A non-specific protease with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.
- Preparing chromosomal DNA for PFGE
- Degrading nucleases during preparation of nucleic acids (includes preparation of native RNA)
Art. No. 7528.3: A stock solution with a concentration of 20 mg/ml can be prepared directly by adding 25 ml buffer or water.
- Subtotal: 0.00
| Order No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 7528.1 | 100 mg | glass | 1 x 100 mg | €72.90 |
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| 7528.2 | 500 mg | glass | 1 x 500 mg | €245.90 |
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| 7528.3 | 500 mg | glass | in 30 ml glass vial | €273.30 |
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| 7528.4 | 1 g | glass | 1 x 1 g | €410.35 |
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| 7528.5 | 250 mg | glass | 1 x 250 mg | €137.40 |
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| 7528.6 | 5 g | glass | 1 x 5 g | €1,627.80 |
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- Subtotal: 0.00
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General information
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Certificates of Analysis
Guarantee analysis
| Activity (Hemoglobin, 37 °C) | ≥30 U/mg |
| DNases, RNases | not detected |
| Endonucleases | not detected |