Jump to content Jump to navigation menu

ROTI®Prep RNA MINI, 250 preparations

for molecular biology
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
warning
H302+H312-H412-EUH032
i harmful if swallowed or in contact with skin, harmful to aquatic life with long lasting effects
P261.1 P273 P301+P312.0 P304+P340
i avoid breathing mist/vapours, avoid release to the environment, IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell, IF INHALED: Remove person to fresh air and keep comfortable for breathing
Pack Qty.
Pack.
Packaging
Density (D) 1,13 g/cm³
Storage temp. +15 to +25 °C
Transport temp. ambient temp.

Kit for RNA isolation from eukaryotic cells, tissues, bacteria, biopsies.
Preparation of up to 100 μg whole RNA from up to 20 mg tissue or cells per column.

Roti®Prep RNA MINI has been developed for the isolation of total RNA from various sou ...
Product details

€858.95/Pack Qty. 

excl. VAT. | 1 kit per Pack Qty.

Art. No. 8485.3

In stock
Free of shipping costs from 125 €
24 hour direct shipment
from 6 Pack Qty. €816.00/Pack Qty.
from 24 Pack Qty. €773.06/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Any questions about this product?

Do you have a question about this product?

In the general information or Product details, we have compiled extensive descriptions and downloads for each product.
If you can not find an answer to your question in the Certificates of Analysis and Application Examples , feel free to contact us!
Contact Service

Product details



ROTI®Prep RNA MINI for molecular biology

Roti®Prep RNA MINI has been developed for the isolation of total RNA from various sources.
The isolation is carried out through the proven and reliable spin column technique, which is very easy to handle and needs only few steps.

  • Preparation by well-known mini spin-column system
  • Fast, easy and efficient
  • Use of DNase not necessary
  • Preparation in approx. 15 to 40 minutes
  • Yields up to 100 μg RNA, depending on source material
  • Elution in 30-80 μl

One of the critical points in RNA extraction is the presence of endogenous RNases, which can lead to high losses very early in extraction of the (in comparison to DNA) very sensitive RNA. Therefores the lysis buffer has been specially optimised to inactivate RNases, with highest efficiency, already at the early stage of the extraction.
Furthermore, in order to get a high-pure total RNA, one particularly for this purpose developed prefilter is used. This filter exclusively serves for binding and targeted removal of DNA impurities, and is discarded after use with the bound DNA. Thus, it is possible to omit application of DNase I.
Total RNA is then obtained from the DNA-free eluate through a simple "Binding-Washing-Elution" process.
In each preparation, up to 100 μg highly pure total RNA may be isolated. One preparation takes (excluding lysis) approx. 10 mins.
Isolated RNA is free of DNA and enzyme inhibitors, and may directly be applied to RT-PCR or other downstream applications.

Possible basic material:
  • Tissues (up to 20 mg)
  • Eukaryotic cells (up to 5x106)
  • Bacteria Gram + and Gram - (up to 1x109)
  • Biopsies
8485_RotiPrepRNA_Ablaufschema

The kit contains

Lysis Buffer LSR, Washing Buffer WSA, Washing Solution WSL, RNase-free Water, spin filters (blue), spin columns (violet), collection tubes, elution tubes, detailed instructions-for-use.
Contents of this Kit may not be bought separately.

Please order ethanol 99,8 % (p.a., Art. No. 9065) and 70 % (p.a., Art. No. T868) separately.



ROTI®Prep RNA MINI
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Packaging Price Quantity
8485.1 1 kit cardboard 10 préparations €57.95
8485.2 1 kit cardboard 50 preparations €223.50
8485.3 1 kit cardboard 250 preparations €858.95
In stock
Available at short notice
Not available
Delivery time on request
Selected quantity:   0
  1. Subtotal:  0.00

Downloads


Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

0 Reviews

0.0 out of five stars