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HPLC column NUCLEODUR® 300-5 C4 ec, 250 mm, 4.6 mm

From  Macherey-Nagel
Particle size
Column length
Column inner diameter


€414.95/Pack Qty. 

excl. VAT. | 1 unit(s) per Pack Qty.

Art. No. 1HKL.1

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Product details



HPLC column NUCLEODUR® 300-5 C4 ec 

Technical Information
Particle size 5 µm
Column length 250 mm
Column inner diameter 4,6 mm
Column volume 4,15 ml
Grid Yes 
Eluent in column Acetonitrile - Water 
Phase 300-5 C4 ec 
Surface chemistry Medium density monomeric butyl (C4) wide-pore modification 
Separation principle Reversed phase (RP) 
Column shape Analytical separation column 
Column type HPLC column 
Particle type Fully porous particles (FPP) 
Specific surface according to BET 100 m²/g
Pore volume 0.9 ml/g
Pore size 300 Å
Temperature stability 60 °C 
Pressure stability 450 bar 
pH stability 1,0-9,0 
Recommended application(s) Biological macromolecules, Peptides, Proteins 
HPLC column NUCLEODUR® 300-5 C4 ec
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Order No. Particle size Column length Column inner diameter Pack Qty. Price Quantity
1HKE.1 5 µm 150 mm 4 mm 1 unit(s) €322.50
1HKH.1 5 µm 150 mm 4.6 mm 1 unit(s) €339.70
1HKK.1 5 µm 250 mm 4 mm 1 unit(s) €392.40
1HKL.1 5 µm 250 mm 4.6 mm 1 unit(s) €414.95
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Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


NUCLEODUR® Columns for HPLC

NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. We offer two phases NUCLEODUR® C18 Gravity which is ideal for sophisticated analytical separations and NUCLEODUR® C18 ec suitable for routine HPLC analysis.


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