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SYBR® Green DNA dye, 9.0 ml, 5 x 1.8 ml

11x conc., for electrophoresis
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Pack Qty.
Pack.
Packaging
Ethidium bromide substitutes
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1

For fluorescent staining of nucleic acids in agarose and polyacrylamide gels.
1.8 ml are sufficient for gel run and staining of approx. 3,600 lanes (5 µl sample + 0.5 µl dye).

This is the DNA dye SYBR® Green I, a staining reagent for detection of double stranded nucleic acid in agarose and polyacrylamide gels. The dye is added directly to the DNA/Loading buffer mixture and then loaded onto the gel.
SYBR® Green I bounds to the minor groove of DNA. Upon excitation it emits a bright green fluorescence. The sensitivity is up to 0.01 ng per band and is therefore 20 times higher than ethidium bromide.
SYBR® Green I has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects.
Product details
Type analysis
€83.75 /Pack Qty.

€62.75/Pack Qty.  Campaign price!

excl. VAT. | 9 ml per Pack Qty.

Art. No. 1CN2.2

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Product details


This is the DNA dye SYBR® Green I, a staining reagent for detection of double stranded nucleic acid in agarose and polyacrylamide gels. The dye is added directly to the DNA/Loading buffer mixture and then loaded onto the gel.
SYBR® Green I bounds to the minor groove of DNA. Upon excitation it emits a bright green fluorescence. The sensitivity is up to 0.01 ng per band and is therefore 20 times higher than ethidium bromide.
SYBR® Green I has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects.

  • Direct addition to DNA sample
  • For excitation with UV light and blue light
  • Non-toxic alternative for ethidium bromide

SYBR® Green DNA dye can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE®PROfessional runVIEW, Art. No. 4849.1). The gels may be documented by all usual broadband ethidium bromide- or SYBR® Green foto filters.


Wavelength

Excitation maximum (bound to DNA): approx. 254 nm and 495 nm
Emission maximum (bound to DNA): 521 nm



SYBR® Green DNA dye 11x conc., for electrophoresis

Technical Information
Max. excitation (DNA-bound) 254 nm
495 nm
Amount of traces ca. 2000/ml 
Emissions max. (DNA-bound) 521 nm 
Sensitivity 0,01 ng/band
SYBR® Green DNA dye
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Order No. Pack Qty. Pack. Packaging Price Quantity
1CN2.1 1.8 ml plastic 1 x 1.8 ml €16.00
1CN2.2 9 ml plastic 5 x 1.8 ml €62.75
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Type analysis

Appearanceorange solution  
Each batch is functionally tested for its suitability in electrophoresis.

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