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4-Methylumbelliferyl-β-D-glucuronide dihydrate, 250 mg

≥99 %, p.a.
Pack Qty.
Empirical formula C16H16O9 · 2 H2O
Molar mass (M) 388,33 g/mol
Melting point (mp) 102 °C
Storage temp. –20 °C
Transport temp. ambient temp.
CAS No. [6160-80-1]
EG-Nr. 228-186-0

Substrate for β-glucuronidase for detection of E. coli.

Ideal for fluorometric detection of β-glucoronidase hydrolysed by MUG to 4-methylumbelliferone.
Detection occurs via long wave UV light (366 nm).
Product details
Type analysis

€67.20/Pack Qty. 

excl. VAT. | 250 mg per Pack Qty.

Art. No. 6394.2

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Product details

4-Methylumbelliferyl-β-D-glucuronide dihydrate ≥99 %, p.a.

Ideal for fluorometric detection of β-glucoronidase hydrolysed by MUG to 4-methylumbelliferone.
Detection occurs via long wave UV light (366 nm).

Technical Information
Enzyme β-D-Glucuronidase 
Detection fluorometric 
Signal product soluble (activity assay) 
4-Methylumbelliferyl-β-D-glucuronide dihydrate
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Order No. Pack Qty. Pack. Price Quantity
6394.1 50 mg glass €24.65
6394.2 250 mg glass €67.20
6394.3 1 g glass €206.95
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General information

Assay protocol for use of BCIP/NBT in immunoblot procedures:

Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.

Detection of peroxidase activity (Immunoassay):

Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos, E.S. et al., (1981) J. Immunoassay 2, (3/4), 187.

Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Assay (titr.)≥99,0 %  
Water (KF)≤10,2 %  
Free 4-methylumbelliferone≤0,0025 %  

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