4-Methylumbelliferyl-β-D-glucuronide dihydrate, 250 mg
Molar mass (M) 388,33 g/mol
Melting point (mp) 102 °C
Storage temp. –20 °C
Transport temp. ambient temp.
CAS No. [6160-80-1]
EG-Nr. 228-186-0
Detection occurs via long wave UV light (366 nm).
€67.20/Pack Qty.
excl. VAT. | 250 mg per Pack Qty.
Art. No. 6394.2
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Product details
4-Methylumbelliferyl-β-D-glucuronide dihydrate ≥99 %, p.a.
Ideal for fluorometric detection of β-glucoronidase hydrolysed by MUG to 4-methylumbelliferone.
Detection occurs via long wave UV light (366 nm).
| Enzyme | β-D-Glucuronidase |
| Detection | fluorometric |
| Signal product | soluble (activity assay) |
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| Order No. | Pack Qty. | Pack. | Price | Quantity | ||
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| 6394.1 | 50 mg | glass | €24.65 |
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| 6394.2 | 250 mg | glass | €67.20 |
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| 6394.3 | 1 g | glass | €206.95 |
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General information
Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.
Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).
Always prepare freshly!
Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.
Reference: Bos, E.S. et al., (1981) J. Immunoassay 2, (3/4), 187.
Certificates of Analysis
Type analysis
| Assay (titr.) | ≥99,0 % |
| Water (KF) | ≤10,2 % |
| Free 4-methylumbelliferone | ≤0,0025 % |