Jump to content Jump to navigation menu

Coelenterazine, 1 mg

≥95 %, for biochemistry
Pack Qty.
Pack.
Empirical formula C26H21N3O3
Molar mass (M) 423,48 g/mol
Melting point (mp) 178 °C
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. [55779-48-1]

Luciferase substrate

Substrate of the Renilla luciferase used for monitoring of the mitochondrial Calcium flux and of rep ...
Product details
Type analysis

€61.20/Pack Qty. 

excl. VAT. | 1 mg per Pack Qty.

Art. No. 4094.3

In stock
Free of shipping costs from 125 €
Delivery fast, simple and reliable!
from 6 Pack Qty. €58.14/Pack Qty.
from 24 Pack Qty. €55.08/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Any questions about this product?

Do you have a question about this product?

In the general information or Product details, we have compiled extensive descriptions and downloads for each product.
If you can not find an answer to your question in the Certificates of Analysis and Application Examples , feel free to contact us!
Contact Service

Product details



Coelenterazine ≥95 %, for biochemistry

Substrate of the Renilla luciferase used for monitoring of the mitochondrial Calcium flux and of reportergenes. Coelenterazine is applied in bioluminescence resonance energy transfer (BRET) assays, ELISAs, HTS and in chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues.



Technical Information
Enzyme Luciferase  
Detection chemiluminescent  
Signal product soluble (ELISA, BRET)  
Coelenterazine
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Price Quantity
4094.3 1 mg glass €61.20
4094.4 2.5 mg glass €114.95
In stock
Available at short notice
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads


General information

Assay protocol for use of BCIP/NBT in immunoblot procedures:

Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.


Detection of peroxidase activity (Immunoassay):

Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos, E.S. et al., (1981) J. Immunoassay 2, (3/4), 187.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Assay (TLC)≥95 %  

0 Reviews

0.0 out of five stars