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Rotitest® Vital

Rotitest® Bio Analysis
Colorimetric test solution for evaluation of cell proliferation and -viability.
1 ml is sufficient for enumeration of 100 cell samples.
Non-cytotoxic cell proliferation test solution.

Rotitest<sup>®</sup> Vital
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Storage temp.: +4 °C
WGK 2

  • Perfectly supersedes MTT
  • Cells keep on being vital
  • Most simple handling
  • One-component-system without radioactivity
  • For adherent and suspension cells
  • Photometric result in 1 to 4 hours
  • Strong correlation between absorbance and cell number
  • Suitable for every culture media

Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays.

In all living cells, NADH and NADPH are formed by the respiratory chain. Roti®-Test vital is based on the (colourless) WST-8, which functions as acceptor for the NADH/NADPH dehydrogenase while being reduced to (coloured) formazane during the process.

The test solution is added directly to the cultivated cells in 96wells. During incubation for few hours, a water-soluble formazan dye is produced by viable cells, and is released into the surrounding medium. The resulting orange colour is measured photometrically (approx. 450 nm). Also suitable for culture media containing phenol red.

Example of use.

Sensitivity of the tetrazolium salt used is higher than that known for standard reagents such as MTT, XTT, MTS, or WST-1. Results were shown to be of highly stringent correlation with [3H]-thymidine incorporation assays, therefore representing viability as well as proliferation status of the cells (Tominaga et al., Anal. Commun. 1999 (36) 47-50).

Fig. A: Correlation of cell counting results achieved with Rotitest® Vital (10 µl/well) and 3H-Thymidine (37 KBq/well). HeLa (1, in DMEM) and HL60 cells (2, in MEM).

Fig. B: Comparison of cell counting results using Rotitest® Vital (10 µl/well) and other cell counting reagents. Photometrical measurement at 450 nm (Rotitest® Vital, XTT), 490 nm (MTS), and 570 nm (MTT). HeLa (1, in DMEM) and HL60 cells (2, in MEM).

Fig. C: Measurement of cytotoxic effects of actinomycin D (exposition 24 h) on HeLa cells (in DMEM), assayed with Rotitest® Vital (10 µl/well).

Fig. D: Proliferation assay on CTLL-2 cells (in RPMI) under influence of humane IL-2 (exposition 72 h), using Rotitest® Vital (10 µl/well).

Each package contains simple-to-follow instructions-for-use.

Stabile at ambient temperature. Very stabile when being cooled.

Store at +4 °C protected from light. Do not freeze.


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Rotitest® Vital

Status Bestellingsnr. Verp. Verpakking Prijs Hoeveelheid Actie
Artikel uit voorraad leverbaar 0069.3 1 ml plastic € 57,95 € 45,15
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Artikel uit voorraad leverbaar 0069.1 5 ml plastic € 166,65 € 129,95
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Artikel uit voorraad leverbaar 0069.2 4 x 5 ml plastic € 504,20 € 393,20
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Technische informatie

Type analysis:
Appearance (test solution)red
Turbiditytransparent
Absorptioncomplies

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