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Synergel™, 100 g

≥99 %, for gel electrophoresis, up to 30 kb
Icon_DNAse-free
Icon_RNAse-free
Verp.
Pack.
Density (D) 1,7 g/cm³
Melting point (mp) 260 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1
CAS No. [9000-40-2]
EG-Nr. 232-541-5

Agarose additive

Type analysis

218,15 €/VE 

Excl. btw | 100 g Per VE

Bestelnr. 0184.1

In stock
Vrij van verzendingskosten vanaf 125 €
24-Uur verzending
Vanaf 6 VE 207,24 €/VE
Vanaf 24 VE 196,34 €/VE
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Productdetails


  • Enhances separation of nucleic acid fragments
  • For extremely clear gels
  • Facilitates image documentation
  • Optimal display especially with small fragments

How to calculate the required Synergel™ quantity?

1. What percentage agarose gel is currently being used?
2. The gel will inevitably contain 0.7 % agarose (the minimum agarose concentration required to ensure gel stability). Hence, subtract 0.7 % from the agarose total.
3. Divide the difference by 2. The result is the percentage of Synergel™ to be added to the 0.7 % agarose.

Example:
Convert a 1 % agarose to a 1 % Synergel™/agarose gel
a. Substract 0.7 % from the 1 % agarose: 1 % - 0.7 % = 0.3 %
b. Divide the difference by 2: 0.3 % : 2 = 0.15 %
c. 0.15 % (150 mg / 100 ml) is the amount of Synergel™ added to 0.7 % agarose(0.7 g / 100 ml) to produce the functional equivalent of a 1 % agarose gel.


Synergel™ ≥99 %, for gel electrophoresis, up to 30 kb

Synergel™ is a synergistic gelling and sieving agent consisting of a modified polysaccharide which, when combined with agarose, forms a hydrogen bonded binary gel system.
The addition of Synergel™ to agarose improves gel performance by providing superior separation and definition of DNA fragments up to 30 kb. A gel mixture containing 0,7 % agarose and 0,7 % Synergel™, e.g., will produce improved results when compared to a 2 % agarose-only gel.
Synergel™ also provides greater optical clarity, which allows higher quality photodocumentation of stained gels.


Gebruiksinstructies

Standard buffer systems like phosphate, acetate or borate buffered Tris-EDTA may be used in forming the gel with agarose/ Synergel™. RNA may also be separated in this system by using a 2,2 M formaldehyde-containing buffer. Synergel™ is compatible with standard blotting procedures, thus, nucleic acid may be electroeluted or transferred to membranes following standard Southern/Northern blotting protocols.


The figure compares separation qualities of
A: 1,5 % Synergel™ / 0,7 % agarose and B: 4 % high-resolution agarose (NuSieve®).
Please see also figures for pUC19 markers T149 and X901.

0184_Bande_ai


Technische informatie
Toepassing Agarose additive for finer pore formation. Increases the separative power of the agarose. For fragments from 10 bp  
Synergel™
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. Verp. Pack. Prijs Hoeveelheid
0184.1 100 g plastic 218,15 €
0184.2 10 g plastic 31,10 €
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Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Colourwhite to off-white powder
Odourslightly salty
pH (1 % solution)9
DNase, RNasenone detected