Synergel™, 100 g
Melting point (mp) 260 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
CAS No. [9000-40-2]
Excl. btw | 100 g Per VE
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- Enhances separation of nucleic acid fragments
- For extremely clear gels
- Facilitates image documentation
- Optimal display especially with small fragments
2. The gel will inevitably contain 0.7 % agarose (the minimum agarose concentration required to ensure gel stability). Hence, subtract 0.7 % from the agarose total.
3. Divide the difference by 2. The result is the percentage of Synergel™ to be added to the 0.7 % agarose.
Convert a 1 % agarose to a 1 % Synergel™/agarose gel
a. Substract 0.7 % from the 1 % agarose: 1 % - 0.7 % = 0.3 %
b. Divide the difference by 2: 0.3 % : 2 = 0.15 %
c. 0.15 % (150 mg / 100 ml) is the amount of Synergel™ added to 0.7 % agarose(0.7 g / 100 ml) to produce the functional equivalent of a 1 % agarose gel.
Synergel™ ≥99 %, for gel electrophoresis, up to 30 kb
Synergel™ is a synergistic gelling and sieving agent consisting of a modified polysaccharide which, when combined with agarose, forms a hydrogen bonded binary gel system.
The addition of Synergel™ to agarose improves gel performance by providing superior separation and definition of DNA fragments up to 30 kb. A gel mixture containing 0,7 % agarose and 0,7 % Synergel™, e.g., will produce improved results when compared to a 2 % agarose-only gel.
Synergel™ also provides greater optical clarity, which allows higher quality photodocumentation of stained gels.
Standard buffer systems like phosphate, acetate or borate buffered Tris-EDTA may be used in forming the gel with agarose/ Synergel™. RNA may also be separated in this system by using a 2,2 M formaldehyde-containing buffer. Synergel™ is compatible with standard blotting procedures, thus, nucleic acid may be electroeluted or transferred to membranes following standard Southern/Northern blotting protocols.
The figure compares separation qualities of
A: 1,5 % Synergel™ / 0,7 % agarose and B: 4 % high-resolution agarose (NuSieve®).
Please see also figures for pUC19 markers T149 and X901.
|Toepassing||Agarose additive for finer pore formation. Increases the separative power of the agarose. For fragments from 10 bp|
- Tussentotaal: 0.00
|0184.1||100 g||plastic||218,15 €||
|0184.2||10 g||plastic||31,10 €||
Levertijd op aanvraag
|Colour||white to off-white powder|
|pH (1 % solution)||9|
|DNase, RNase||none detected|