Synergel™, 100 g
Melting point (mp) 260 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
CAS No. [9000-40-2]
Excl. btw | 100 g Per VE
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- Enhances separation of nucleic acid fragments
- For extremely clear gels
- Facilitates image documentation
- Optimal display especially with small fragments
Synergel™ ≥99 %, for gel electrophoresis, up to 30 kb
Synergel™ is a synergistic gelling and sieving agent consisting of a modified polysaccharide which, when combined with agarose, forms a hydrogen bonded binary gel system.
The addition of Synergel™ to agarose improves gel performance by providing superior separation and definition of DNA fragments up to 30 kb. A gel mixture containing 0,7 % agarose and 0,7 % Synergel™, e.g., will produce improved results when compared to a 2 % agarose-only gel.
Synergel™ also provides greater optical clarity, which allows higher quality photodocumentation of stained gels.
Standard buffer systems like phosphate, acetate or borate buffered Tris-EDTA may be used in forming the gel with agarose/ Synergel™. RNA may also be separated in this system by using a 2,2 M formaldehyde-containing buffer. Synergel™ is compatible with standard blotting procedures, thus, nucleic acid may be electroeluted or transferred to membranes following standard Southern/Northern blotting protocols.
The figure compares separation qualities of
A: 1,5 % Synergel™ / 0,7 % agarose and B: 4 % high-resolution agarose (NuSieve®).
Please see also figures for pUC19 markers T149 and X901.
|Toepassing||Agarose additive for finer pore formation. Increases the separative power of the agarose. For fragments from 10 bp|
- Tussentotaal: 0.00
|0184.1||100 g||plastic||225,25 €||
|0184.2||10 g||plastic||32,15 €||
Leveringsdatum onbekend op dit moment
|Colour||white to off-white powder|
|pH (1 % solution)||9|
|DNase, RNase||none detected|