Transfer membrane Amersham™ Protran® supported 0.45 μm NC supported sheet 20 × 20 cm
Excl. btw | 25 blad Per VE
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Transfer membrane for most nucleic acid and protein immobilising techniques
Multiple use - ideal for most nucleic acid and protein immobilising techniques. Pure nitro-cellulose, free from cellulose acetate and triton. They have a high binding capacity and low background. The fibre-reinforced version allows extensive handling of membrane, easier cutting, repeated hybridisation and automated immobilising.
Nitrocellulose should only conditionally be used for electrophoretic transfer of nucleic acids, because of the high salt concentrations required for the transfer.
Membranes of 0,45 μm pore size allow for DNA transfer (minimum 300 bp) and protein transfer (minimum 20 kDa).
Southern, Northern, Western transfer, colony and plaque hybridisation, immunoblots, nucleic acid and protein dot-blot, slot-blot, electrophoretic transfer of proteins.
Detection systems are radioactivity, chemoluminescence, colour
Transfer membrane Amersham™ Protran® supported 0.45 μm NC supported sheet 20 × 20 cm Amersham™ Protran®
Replaces Protran® BA-S 85 from Whatman®
Reinforced with a synthetic, inert material for improved elasticity and reaction to wetness. Suitable for protein and nucleic acid transfers which require a better resistance to wetness, such as repeated screening of the same blot.
|Binding capacity||86–110 µg/cm²|
|Immobilization||UV, baking (max. 80 °C)|
- Tussentotaal: 0.00
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