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Transfer membrane ROTI®Nylon 0.2, 0,45 µm, Ø8.2 cm

Made of Nylon 6.6.

Ideal for DNA/RNA transfer, particularly for colony- and plaque-lifting, as well as for all multi ...

Excl. btw | 1 Verpakking Per VE

Bestelnr. HP38.1

Not available
Vrij van verzendingskosten vanaf 125 €
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Made of Nylon 6.6.

Ideal for DNA/RNA transfer, particularly for colony- and plaque-lifting, as well as for all multiple rehybridisations.
Especially recommended for Northern-blots.

ROTI®Nylon 0.2 is a neutral, enhanced Nylon membrane. Based on the material it provides a superior tensile strength and is extremely resistant against alcohol, acetone, and many other organic solvents. I

n a buffer pH of 6,5 ROTI®Nylon 0.2 is uncharged and by means of the surface-standing amino- and carboxy-groups has superior hydrophilic qualities. The charge of the membrane (ζ-potential) may be altered through the pH of the buffer - the membrane is positively charged at pH <6,5 and negatively charged at pH >6,5.
Membrane of particulary high DNA-binding capacity. Available as roll (pore size 0,2 µm) for high-flexible usage, and as disks (pore size 0,45 µm) for colony- and plaque-lifting. May be steam sterilised.
Covalent bondage of transferred nucleic acids is achieved by cross linking (baking for 15 min at 80 °C or UV-cross linking for 20 sec at 260 nm). Due to the excellent signal-to-noise ratio of the membrane, very sensitive detection (single copy-gene detection) and multiple rehybridisation is possible without loosing sensitivity.

Southern- and Northern blot, Dot blot, DNA fingerprinting, ELISA, colony- and plaque lifting, macro- and microarrays.
Detection systems: radioactivity, chemoluminescence, fluorescence

Technische informatie
Uitvoering disks  
Poriëngrootte 0,45  µm
Type 0,45 µm  
Lading neutral/amphoteric  
Binding capacity 500  µg/cm²
Dikte 150±20  µm
Immobilization UV, baking  
Transfer membrane ROTI®Nylon 0.2
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Poriëngrootte Prijs Hoeveelheid
HP38.1 1 Verpakking 0,45 µm
AE50.1 1 rol(len) 0,2 µm 320,35 €
Op voorraad
Binnenkort beschikbaar
Niet beschikbaar
Levertijd op aanvraag
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00


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