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ROTI®Phenol/ Chloroform/ Isoamyl alcohol, 500 ml

for the extraction of nucleic acids
Icon_EVE-EUD Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
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Mogelijke gevolgen: vernietigen metalen en zijn bijtend voor lichaamsweefsel; ernstig oogletsel is mogelijk. Veiligheid: contact vermijden; veiligheidsbril en handschoenen dragen. Bij contact ogen en huid met water spoelen.
Mogelijke gevolgen: leiden in kleinere hoeveelheden onmiddellijk tot ernstige schade aan de gezondheid of tot de dood. Veiligheid: niet inademen, aanraken, inslikken. Beschermingsmiddelen dragen. Onmiddellijk antigifcentrum of arts op de hoogte brengen. Stabiele zijpositie.
Mogelijke gevolgen: veroorzaken allergieën, kankerverwekkend (carcinogeen), verandering erfelijk materiaal (mutageen), brengt de voortplanting in gevaar en vruchtbeschadigend (reprotoxisch) of orgaanbeschadigend Veiligheid: voor het werk met dergelijke stoffen moet men zich goed informeren; veiligheidskleding en handschoenen, oog- en mondbescherming of adembescherming dragen.
Mogelijke gevolgen: zijn voor waterorganismen schadelijk, giftig of zeer giftig, acuut of met langdurige werking. Veiligheid: alleen met gevaarlijk afval afvoeren, in geen geval in het milieu laten terechtkomen.
danger
H301+H331-H312-H314-H341-H351-H361d-H372-H411
i toxic if swallowed or if inhaled, harmful in contact with skin, causes severe skin burns and eye damage, suspected of causing genetic defects, suspected of causing cancer, suspected of damaging the unborn child, causes damage to organs through prolonged or repeated exposure, toxic to aquatic life with long lasting effects
P201 P270 P280 P304+P340 P305+P351+P338 P308+P313
i obtain special instructions before use, do not eat, drink or smoke when using this product, wear protective gloves/protective clothing/eye protection/face protection, IF INHALED: Remove person to fresh air and keep comfortable for breathing, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, IF exposed or concerned: Get medical advice/attention
Verp.
Pack.
Phenol solutions
Density (D) >1,2 g/cm³
Boiling point (bp) >65 °C
Storage temp. +4 °C
ADR 6.1 II
WGK 3
UN-Nr. 2821

Redistilled, in TE‑buffer equilibrated phenol, chloroform and isoamyl alcohol at a ratio of 25:24:1, pH 7,5-8,0.
Draw solution from lower phase. Do not shake before use!
Covered with buffer. Store in the dark!

ROTI®Phenol/Chloroform/Isoamyl alcohol is re-distilled phenol, equilibrated in TE buf ...
Productdetails

128,90 €/VE 

Excl. btw | 500 ml Per VE

Bestelnr. A156.2

Op voorraad
Vrij van verzendingskosten vanaf 125 €
Snelle en betrouwbare levering! (Door de grote vraag kan er een vertraging van maximaal 5 werkdagen ontstaan in de verzending.)
Vanaf 6 VE 122,46 €/VE
Vanaf 24 VE 116,01 €/VE
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Productdetails


ROTI®Phenol

ROTI®Phenol solutions for DNA and RNA extraction

• Reduce exposure to toxic chemicals
• Prepared from phenol of highest purity
• Packed under argon for maximum stability
• Successfully tried and tested in many researchlaboratories

Please note our Technical Information Brochure on the Internet next to the products:

Phenolic DNA Isolation
Background and protocol

Mechanism

Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses.
For distinct phase separation, ROTI®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl.

Draw solution from lower phase. Do not shake before use!


ROTI®Phenol/ Chloroform/ Isoamyl alcohol for the extraction of nucleic acids

ROTI®Phenol/Chloroform/Isoamyl alcohol is re-distilled phenol, equilibrated in TE buffer, premixed with high purity chloroform and isoamyl alcohol.

ROTI®Phenol/Chloroform/Isoamyl alcohol has proven itself in many research laboratories for the isolation of nucleic acids and is an ideal complement to pure ROTI®Phenol.


Mechanisme

Addition of 1 volume ROTI®Phenol/Chloroform/Isoamyl alcohol and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase.



ROTI®Phenol/ Chloroform/ Isoamyl alcohol
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. Verp. Pack. Prijs Hoeveelheid
A156.1 250 ml glass 77,30 €
A156.2 500 ml glass 128,90 €
A156.3 100 ml glass 39,25 €
Op voorraad
Leveringsdatum onbekend op dit moment
Niet beschikbaar
Levertijd op aanvraag
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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