061 - 712 11 60 (Switzerland)

Ponceau S (C.I. 27195)

for histology and electrophoresis
For reversible protein staining on membranes and for microscopy.
Scarlet red

Ponceau S (C.I. 27195)
Empirical formula C22H12N4Na4O13S4
Molar mass (M) 760,56 g/mol
Solubility 10 g/l (H2O, 20 °C)
WGK 1
CAS No.[6226-79-5]
EG-Nr. 228-319-2

Compatible with PVDF and NC membranes.

Staining of NC membranes: 0.2-2 % Ponceau S in 3 % trichloroacetic acid (Art. No. 8789.1) + 3 % sulphosalicylic acid (Art. No. 4119.1), incubate for 1-5 mins. at room temperature. Enhance contrast by incubation in water acidified by a few drops acetic acid.

Staining of PVDF membranes: 0.1-1 % Ponceau S in 10 % acetic acid (Art. No. 6755.1), incubate 1-5 mins. at room temperature. Ponceau S stained bands on PVDF only appear bright and distinct after drying (not recommended if Western blotting shall be performed).

Prior to Western blot analysis decolourise in PBS (Art. No. 1058.1) or TBS (Art. No. 1060) for 10-20 mins. at room temperature.


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Warning H315-H319-H335

Ponceau S (C.I. 27195)

Status Art. No. Pack Qty. Packaging Price Quantity Action
Item available in stock 5938.2 10 g glass CHF 34.90
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Item available in stock 5938.1 25 g glass CHF 69.90
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Technical information

Type analysis:
Absorption max. (in H2O)517-523 nm
Specific extinction E 1 %/1 cm>460
Loss on drying (110 °C)≤10 %

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