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Denhardt's solution, lyophilised powder

50x, BioScience Grade, for molecular biology
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Storage temp. +4 °C
WGK 3

Blocking reagent for hybridisation and stripping.
The bottle contains powder mix for 50 ml 50x stock solution. Add 48,8 ml water to gain exactly 50 ml stock solution. Sterilise stock solution by filtration (0,2 µm) and store in aliquots of 10 ml at -20 °C .

Denhardt's lyophilised powder contains a mixture of different blocking agents that is used for nucle ...
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CHF135.10/Pack Qty. 

excl. VAT. | 50 ml per Pack Qty.

Art. No. HP33.1

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Denhardt's solution, lyophilised powder 50x, BioScience Grade, for molecular biology

Denhardt's lyophilised powder contains a mixture of different blocking agents that is used for nuclear acid hybridisation (Northern and Southern Blot) as well as for stripping of hybridised membranes. By prehybridisation the filter membranes with Denhardt's solution unspecific binding of DNA to the membrane is avoided.


Directions for use

For hybridisation use 10x Denhardt's solution with 6x SSC (ROTI®Stock 20x SSC, Art. No. 1054.1) supplemented with 100 µg/ml denatured salmon sperm DNA and 1 % SDS (ROTI®Stock 20 % SDS, Art. No. 1057.1).



Denhardt's solution, lyophilised powder
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HP33.1 50 ml plastic Powder for 50 ml CHF135.10
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Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Albumin Fraction V0,5 g  
Ficoll 4000,5 g  
Polyvinylpyrrolidone K300,5 g  
pH-value (stock solution in water)6,3 ±0,2  

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