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Transfer membrane ROTI®FluoroPVDF, 20 × 13 cm

From  ROTH SELECTION
Material: Polyvinylidene fluoride (PVDF).
Dimensions


Premium PVDF membrane from Carl ROTH, suitable for direct fluorescence detection

ROTI®Fluoro PVDF is a white, microporous membrane made from naturally hydrophobic polyvinylidene fluoride. The membrane is of superior protein binding capacity, making it perfectly suited for all standard and special applications in the field of protein analysis.
Product details

CHF43.00/Pack Qty. 

excl. VAT. | 2 sheet per Pack Qty.

Art. No. 2831.1

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from 6 Pack Qty. CHF40.85/Pack Qty.
from 24 Pack Qty. CHF38.70/Pack Qty.
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Product details

Material: Polyvinylidene fluoride (PVDF).

Premium PVDF membrane from Carl ROTH, suitable for direct fluorescence detection

ROTI®Fluoro PVDF is a white, microporous membrane made from naturally hydrophobic polyvinylidene fluoride. The membrane is of superior protein binding capacity, making it perfectly suited for all standard and special applications in the field of protein analysis.


Directions for use

ROTI®Fluoro PVDF has been specially optimised for direct detection via fluorescence.
ROTI®Fluoro PVDF binds proteins adhesively via hydrophobic interactions. It enables very sensitive protein detection by keeping the background very low, resulting in a good signal-to-noise ratio with all standard detection systems. The mechanically rigid membrane is of high tensile strength and tear-proof, and does not crack or curl during handling. Therefore, signals remain clear and distinct, and are undisturbed by false-positive signals caused by membranes crinkles.
Briefly immerse ROTI®Fluoro PVDF membrane in 100 % methanol prior to transfer until no more white areas are visible. This transfer membrane is then compatible with every aqueous transfer system.

The membrane ROTI®Fluoro PVDF is only conditionally suitable for use with chemiluminescence detection. The membranes are specially coated for fluorescence applications and this coating can reduce the sensitivity of chemiluminescence. For chemiluminescent assays we recommend the use of ROTI®PVDF membranes.


Application examples

Western- and Far-Western-Blot, analysis of small peptides, Immunoblot, Dot-blot, binding assays, N-terminal sequencing
Detection systems: fluorescence, radioactivity, colour"



Transfer membrane ROTI®FluoroPVDF 

Technical Information
Dimensions 20 × 13 cm 
Version sheet 
Pore size 0,2 µm
Bond hydrophobic 
Binding capacity 300 µg/cm²
Thickn. 125±25 µm
Immobilisation not necessary 
Transfer membrane ROTI®FluoroPVDF
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Order No. Dimensions Pack Qty. Price Quantity
2831.1 20 × 13 cm 2 sheet CHF43.00
2803.1 330 × 26 cm 1 roll(s) CHF450.80
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