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Ponceau S (C.I. 27195), 25 g

for histology and electrophoresis
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H315-H319-H335
i causes skin irritation, causes serious eye irritation, may cause respiratory irritation
P260 P302+P352 P305+P351+P338
i do not breathe dust, IF ON SKIN: Wash with plenty of water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Scarlet red
Empirical formula C22H12N4Na4O13S4
Molar mass (M) 760,56 g/mol
WGK 1
CAS No. [6226-79-5]
EG-Nr. 228-319-2

For reversible protein staining on membranes and for microscopy.
Suitable for NC and PVDF membranes as well as prior to Western analyses.

Directions for use
Product details
Type analysis
CHF72.70 /Pack Qty.

CHF56.60/Pack Qty.  Campaign price!

excl. VAT. | 25 g per Pack Qty.

Art. No. 5938.1

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Ponceau S (C.I. 27195) for histology and electrophoresis
Directions for use

Staining of NC membranes: 0.2-2 % Ponceau S in 3 % trichloroacetic acid (Art. No. 8789.1) + 3 % sulphosalicylic acid (Art. No. 4119.1), incubate for 1-5 mins. at room temperature. Enhance contrast by incubation in water acidified by a few drops acetic acid.

Staining of PVDF membranes: 0.1-1 % Ponceau S in 10 % acetic acid (Art. No. 6755.1), incubate 1-5 mins. at room temperature. Ponceau S stained bands on PVDF only appear bright and distinct after drying (not recommended if Western blotting shall be performed).

Prior to Western blot analysis decolourise in PBS (Art. No. 1058.1) or TBS (Art. No. 1060) for 10-20 mins. at room temperature.



Ponceau S (C.I. 27195)
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5938.1 25 g glass CHF56.60
5938.2 10 g glass CHF27.85
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Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Absorption max. (in H2O)517-523 nm  
Specific extinction E 1 %/1 cm>460  
Loss on drying (110 °C)≤10 %  

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