Molar mass (M) 418,40 g/mol
Melting point (mp) 150 °C
Storage temp. +4 °C
CAS No. [5133-19-7]
excl. VAT. | 50 mg per Pack Qty.
Art. No. 5555.1
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Although the principle of thin-layer chromatography is more than a century old, it did not make its breakthrough as an analytical method until about 50 years ago.
Thanks to the development of new sorbents and supports, as well as increasing instrumentation and automation, TLC has become a versatile separation method. It is used both in qualitative analysis and in quantitative analysis.
Applications range from simple manual separation processes in classic TLC to automated processes in HPTLC (high performance thin layer chromatography).
Advantages of thin layer chromatography:
- Higher sample throughput in less time
- Suitable for screening tests
- Pilot process for HPLC
- The ready-to-use TLC layer functions as a data storage device for separation results
- The separated substances can be used later for further analysis (e.g. IR, MS)
- By switching the mobile and the stationary phases, the separation process can be optimised quickly and cost-efficient
Pure reference substances are essential for precise analysis. They are used for identifying compounds by retention time, for calibrating detectors, and for eliminating errors in quantification of the sample compounds.
Peak areas are proportional to sample quantity in all chromatographic methods. For precise quantitative analysis, however, it is necessary to calibrate the detector as the signal may significantly vary with different substances.
To avoid errors during sample preparation and injection, an internal standard should be used in any case. The standard must not be present in the sample, however, its chemical and physical properties ought to be the closest possible to the analytical targets. It must be well resolved in the chromatogram within the same range of retention time as the sample. Before sample analysis, a given volume of standard is added, and all clean-up procedures are made with the sample containing the standard. Quantitative errors may be compensated by comparing the peak-areas of the sample with that of the internal standard.
Reference Substances for TLC
ROTICHROM® TLC comparison substances are supplied with a certificate of analysis and a description of the chromatographic method, enclosed in the outer package.