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DEAE Dextran, 25 g

for biochemistry
Pack Qty.
Molar mass (M) ~500000 g/mol
CAS No. [9013-34-7]

Transfection reagent

DEAE is the oldest transfection reagent known. The method of complexation of plasmid DNA with diethy ...
Product details
Type analysis

CHF78.30/Pack Qty. 

excl. VAT. | 25 g per Pack Qty.

Art. No. 4198.1

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Product details

DEAE Dextran for biochemistry

DEAE is the oldest transfection reagent known. The method of complexation of plasmid DNA with diethylaminoethyldextran has been published in 1968 by McCutchan. Positively charged DEAE binds negatively charged DNA to aggregates, which then bind to negtively charged surface structures of cell membranes. Uptake is done via endocytosis. This DEAE method needs only little amounts of DNA, while resulting in high transfection efficiency of up to 30 %. However, the cytotoxic effects caused by DEAE allow only transient transfections. The following cell lines are well transfectable: CV-1, BSC-1 and COS cells, and other stabile, insensitive cell lines.

Stock solution: 50 mg/ml in H2O. Autoclave.

End concentration: 1 mg/ml (transfection for 30-90 min.) or 250 µg/ml (transfection for up to 8 h)

DEAE Dextran
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Order No. Pack Qty. Pack. Price Quantity
4198.1 25 g plastic CHF78.30
4198.2 100 g plastic CHF248.50
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Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Appearancewhite to creamcoloured powder  
Loss on drying≤10 %  
pH value (5 % in water)5,0-7,0  
Nitrogen content (N)3,0-3,8 %  
Sulphated ash≤2 %  
Free DEAE-Cl≤0,002 %  

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