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ROTI®Pol TaqS Red-Mix (2x), 2.5 ml, 1 x 2,5 ml

2x konz., ready-to-use
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Pack Qty.
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PCR master mix
Density (D) 1 g/cm³
Storage temp. -20 °C
Transport temp. cooled
WGK 1

Taq master mix for standard PCR amplifications followed by direct gel-loading.
Prestained PCR premix w/o primers and template, optimized for reactions of 25 µl volume.

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticu ...
Product details
Type analysis

CHF62.90/Pack Qty. 

excl. VAT. | 2.5 ml per Pack Qty.

Art. No. 9241.1

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Product details


For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.


ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.



ROTI®Pol TaqS Red-Mix (2x) 2x konz., ready-to-use

Optimized pre-mixed 2x PCR solution containing the recombinant TaqS DNA polymerase from the thermophilic bacterium Thermus aquaticus, dNTPs, MgCl2, and all other components required for PCR (w/o primers and template DNA), plus components for direct gel electrophoresis. ROTI®Pol TaqS Red-Mix (2x) is recommended for use in all standard PCR applications immediately followed by gel electrophoresis.

PCR assaying with ROTI®Pol TaqS Red-Mix (2x) master mix not only reduces contamination risks, but is also time-saving, highly reproducible and very easy to prepare. ROTI®Pol TaqS Red-Mix (2x) can be applied to all standard Taq-based cycling protocols and is, therefore, the optimal choice for high throughput PCR as being performed in, for instance, analysis of cloning efficiency or colony screening, for routine screening processes, and for student's courses.

Due to the optimized composition of the master mix, the TaqS polymerase delivers specific PCR amplification of good yield with a wide range of PCR templates. ROTI®Pol TaqS Red-Mix (2x) is able to amplify PCR products up to 3 kb with genomic DNA, and is appropriate for use with pure DNA solutions, cDNA, and bacterial colonies as templates. In 1 % agarose gels, the included red dye migrates approx. as fast as a 1 kb DNA fragment. During denaturation in Southern blotting, the dye turns yellow at an acidic pH. The TaqS polymerase included in the master mix possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.

RotiPol-Sensitivitaetsassay
Figure: Sensitivity assay using ROTI®Pol TaqS, TaqHY, Hot-TaqS and Hot-TaqHY, 1 U/reaction each (20 μl). 300 bp β-Actin fragment, 40 cycles. Template: 10 to 2000 pg human gDNA. Gel loading 10 μl each.
M: 100 bp-DNA Ladder extended. NTC: no template control.

The set contains

2 or 10 vials, respectively, with 1,25 ml each of ROTI®Pol TaqS Red-Mix (2x) containing TaqS polymerase, 0,4 mM each dNTP, and 4 mM MgCl2 in 2x reaction buffer with 0,02 % cresol red.



Technical Information
Use Standard PCR with subsequent gel loading  
Amplicon ends 3'dA  
Master-Mix red (ready to load)  
Polymerase Standard Taq polymerase  
Use Standard PCR with subsequent gel loading  
ROTI®Pol TaqS Red-Mix (2x)
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Order No. Pack Qty. Pack. Packaging Price Quantity
9241.1 2.5 ml plastic 1 x 2,5 ml CHF62.90
9241.2 12.5 ml plastic 5 x 2,5 ml CHF250.60
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Downloads


Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearancered
Endonuclease activitynone detected
Exonuclease activitynone detected
Suitability for PCR (400 bp)complies
Suitability for PCR (3 kb)complies
Selective specificity in PCRcomplies
For research use only. Not approved for use in clinical or in vitro diagnostics.