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ROTI®Pol TaqS, 100 µl, 1 x 500 U

5 U/μl
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Pack Qty.
Pack.
Packaging
Taq DNA polymerase, recombinant, DNA polymerase (thermostable), Polymerase (thermostable)
Storage temp. -20 °C
Transport temp. cooled
WGK 1

For all standard PCR amplifications.
TaqS polymerase in storage buffer, PCR buffer (10x), PCR buffer red (10x)

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticu ...
Product details
Type analysis

CHF81.10/Pack Qty. 

excl. VAT. | 100 µl per Pack Qty.

Art. No. 9223.1

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Product details


For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.


ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.



ROTI®Pol TaqS 5 U/μl

Recombinant full-length form of the heat stable Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus in storage buffer, plus additional 10x concentrated PCR reaction buffer and 10x concentrated PCR reaction buffer with red gel loading dye. ROTI®Pol TaqS is recommended for all standard PCR applications.

This polymerase set ROTI®Pol TaqS is the optimal choice for all standard Taq-based cycling protocols, as being performed in, for instance, analysis of cloning efficiency, routine screening processes, educational assays and much more. In combination with our unique buffers, the TaqS polymerase delivers specific PCR amplification of good yield with a wide range of PCR templates.

ROTI®Pol TaqS is able to amplify PCR products up to 3 kb with genomic DNA and is appropriate for use in the amplification of DNA from genomic, viral, and plasmid templates. The TaqS DNA polymerase included in the set possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.

RotiPol-Sensitivitaetsassay
Figure: Sensitivity assay using ROTI®Pol TaqS, TaqHY, Hot-TaqS and Hot-TaqHY, 1 U/reaction each (20 μl). 300 bp β-Actin fragment, 40 cycles. Template: 10 to 2000 pg human gDNA. Gel loading 10 μl each.
M: 100 bp-DNA Ladder extended. NTC: no template control.

Filled in colour coded tubes, the set contains the DNA polymerase and two 10x concentrated reaction buffers with 20 mM MgCl2, one of which has been specially designed for direct gel loading following the PCR reaction. In 1 % agarose gels, the included red dye migrates approx. as fast as a 1 kb DNA fragment. During denaturation in Southern blotting, the dye turns yellow at an acidic pH. The use of the colourless PCR reaction buffer is adequate for all general PCR applications and is particularly recommended when direct fluorescence or absorbance readings are required.


The set contains

TaqS polymerase (Art. No. 0518) in storage buffer containing 50 % glycerol, PCR buffer (10x) (Art. No. 0511) with 20 mM MgCl2, PCR buffer red (10x) (Art. No. 0527) with 20 mM MgCl2 and 0,1 % cresol red.
Colour coded tubes.
Contents of this set may not be bought separately.



Technical Information
Use Standard PCR  
Amplicon ends 3'dA  
Polymerase Standard Taq polymerase  
Reaction buffer colourless + red (ready to load)  
Use Standard PCR  
ROTI®Pol TaqS
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Order No. Pack Qty. Pack. Packaging Price Quantity
9223.1 100 µl plastic 1 x 500 U CHF81.10
9223.2 500 µl plastic 5 x 500 U CHF385.00
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Downloads


Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Purity (enzyme)≥98%
Enzyme activity5 U/µl
Endonuclease activitynone detected
Exonuclease activitynone detected
Suitability for PCR (400 bp)complies
Suitability for PCR (3 kb)complies
Selective specificity in PCRcomplies
Stability (in storage buffer)complies
For research use only. Not approved for use in clinical or in vitro diagnostics.