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Biotin-11-dUTP, 25 µl, 25 nmol

≥95 %, 1 mM solution
Pack Qty.
Empirical formula C28H42N6O17P3S
Molar mass (M) 859,65 g/mol
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. cooled

For non-radioactive, enzymatic labelling of DNA.

CHF163.10/Pack Qty. 

excl. VAT. | 25 µl per Pack Qty.

Art. No. 1047.1

In stock
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from 6 Pack Qty. CHF154.94/Pack Qty.
from 24 Pack Qty. CHF146.79/Pack Qty.
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Product details

  • DNAse-, RNAse-, Protease- and Phosphatase-free
  • Free of PCR inhibitors like modified bases and tetra-pyrophosphate
  • Adjusted to pH 7,5 for optimzed enzyme compatibility
  • Highly efficient enzymatic fabrication
  • Can be optimally combined with Carl ROTH ROTI®Pol DNA polymerases

Suitable for

PCR, cDNA synthesis, labelling and primer-extension, mutagenesis-assays, sequencing reactions and in vitro transcription.

All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also ’long range PCR’, repeated quantitative light-cycling reactions, and tests for physical stability.

Biotin-11-dUTP ≥95 %, 1 mM solution

Biotin-11-dUTP is able to replace dTTP in growing DNA-strands and is used for efficient non-radioactive DNA-labelling. As a general rule, the labelled DNA is then detected using streptavidin-conjugates by colorimetric (e.g. streptavidin-AP) or fluorometric (e.g. fluorescein-streptavidin) assays.
Biotin-11-dUTP can be used for double staining techniques in combination with rhodamine-12-dUTP or fluorescein-12-dUTP and corresponding antibodies.

Applications: Non-radioactive labelling of DNA by enzymatic reactions, e.g. PCR, reverse transcription, nick-end-translation, end-labelling or random-primed DNA-labelling. Incorporation can be done with all established DNA-polymerases (e.g. Taq-polymerase, T4 DNA-polymerase, Klenow fragment).
Testet for the lack of endo-, exodeoxyribonuclease, ribonuclease and phosphatase.

ε240 = 10,7 E x mmol-1 x cm-1; ε289 = 7,1 E x mmol-1 x cm-1; pH: 7,5 ±0,2

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Order No. Pack Qty. Pack. Packaging Price Quantity
1047.1 25 µl plastic 25 nmol CHF163.10
1047.2 50 µl plastic 50 nmol CHF274.30
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