Proteinase K, 100 mg, 1 x 100 mg
CAS No. [39450-01-6]
Art. No. 7528.3: A The stock solution (20 mg/ml) can be prepared directly in the bottle.
excl. VAT. | 100 mg per Pack Qty.
Art. No. 7528.1
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Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNase and DNase not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.
Stock solution: 20 mg/ml in water
Storage temperature (stock solution): -20 °C
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
Working solution: 50 µg/ml
Proteinase K ≥30 mAnson U/mg, lyophilized
A non-specific protease with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.
- Preparing chromosomal DNA for PFGE
- Degrading nucleases during preparation of nucleic acids (includes preparation of native RNA)
Art. No. 7528.3: A stock solution with a concentration of 20 mg/ml can be prepared directly by adding 25 ml buffer or water.
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|7528.1||100 mg||glass||1 x 100 mg||CHF126.70||
|7528.2||500 mg||glass||1 x 500 mg||CHF427.00||
|7528.3||500 mg||glass||for direct preparation of stock sol.||CHF474.60||
|7528.4||1 g||glass||1 x 1 g||CHF712.60||
|7528.5||250 mg||glass||1 x 250 mg||CHF238.70||
|7528.6||5 g||glass||1 x 5 g||CHF2,826.60||
Available at short notice
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Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
|Activity (Hemoglobin, 37 °C)||≥30 U/mg|
|DNases, RNases||not detected|