Jump to content Jump to navigation menu

Zymolyase® 20T, 100 mg

≥20 U/mg, for biochemistry and molecular biology
Icon_Eisstern All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Pack Qty.
Lyticase, β-1,3-glucan laminaripentaohydrolase
Storage temp. +4 °C
Transport temp. cooled
CAS No. [37340-57-1]

For lysis of yeast cells. Isolated from Arthrobacter luteus.
Please note: Zymolyase® does not dissolve completely in higher concentrations.

Zymolyase®, produced by a submerged culture of Arthrobacter luteus, has strong ...
Product details
Type analysis

CHF107.70/Pack Qty. 

excl. VAT. | 100 mg per Pack Qty.

Art. No. 9324.1

In stock
Free of shipping costs from 200 CHF
Delivery fast, simple and reliable!
from 6 Pack Qty. CHF102.32/Pack Qty.
from 24 Pack Qty. CHF96.93/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:

Put all products for your subscription in the desired quantity in the basket.


In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.


Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Any questions about this product?

Do you have a question about this product?

In the general information or Product details, we have compiled extensive descriptions and downloads for each product.
If you can not find an answer to your question in the Certificates of Analysis and Application Examples , feel free to contact us!
Contact Service

Product details

Zymolyase®, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living cell walls of various strains of yeast cells. Hence, it is frequently used in order to produce protoplasts or spheroplasts.

The essential enzyme for the lytic activity of Zymolyase® is β‑1,3‑glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit.

Other enzymatic activities contained: β-1,3-glucanase, protease, mannanase. Trace amounts of amylase, xylanase, phosphatase.

Stability: At 30 °C 70 % of the lytic activity is lost after 3 months.

Optimum temperature and pH: at pH 7,5 - 35 °C for lysis of viable yeast cells, at pH 6,5 - 45 °C for hydrolysis of yeast glucan.

Specificity (lytic spectrum): Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschnikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schwanniomyces, etc.

One unit of lytic activity is defined as the enzyme amount causing a decrease of 30 % in absorbance at 800 nm using 6 mg Brewer's yeast as substrate in Phosphate buffer (pH 7,5) at 25 °C. Lytic activity varies depending on the particular yeast strain, the growth stage of the yeast, and cultural conditions.

Zymolyase® 20T ≥20 U/mg, for biochemistry and molecular biology
Directions for use

As stock solution, a 2 to 10 % (20 or 100 mg/ml) Zymolyase® solution may be prepared, solubilizing the enzyme in water, 10 mM sodiumphosphate buffer (pH 7,4) or 50 mM Tris-Cl (pH 7,5), respectively, according to the buffer system to be used downstream and containing 5 % glucose and 50 % glycerol each. The Zymolyase® tends to not dissolve completely. Do not heat for solubilization, but rather use the Zymolyase® as suspension. The suspension may be stored in aliquots at -20 °C. For use in the respective buffer system, dilute the stock solution to the working concentration of 2-5 mg/ml and optionally sterilize by filtration (0,2 µm).
In most cases, however, Zymolyase® is solubilized in freshly prepared working buffer (for instance rescue buffer 50 mM Tris-Cl, pH 7,5, 10 mM EDTA, 0,3 % β-Mercaptoethanol) in the required working concentration directly prior to use.
Avoid using nitrocellulose filters when sterilizing - Zymolyase® may be adsorbed to nitrocellulose membranes.

Zymolyase® 20T is prepared by ammonium sulfate precipitatation.

Zymolyase® 20T
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Price Quantity
9324.1 100 mg glass CHF107.70
9324.2 500 mg glass CHF306.60
9324.3 1 g glass CHF539.00
In stock
Available at short notice
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00


General information

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
Oxidoreductases (catalyse redox reactions)
Transferases (transfer functional groups among substrates)
Hydrolases (cleave bonds via addition of water)
Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
Isomerases (transform chemical isomers)
Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)

Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearancelyophilised powder  
Activity≥20 000 U/g  

0 Reviews

0.0 out of five stars