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Ficoll® 400, 25 g

for molecular biology and biochemistry
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Non-ionic polymer of saccharose
Molar mass (M) ~400 000 g/mol
CAS No. [26873-85-8]

For density gradient centrifugation; ingredient of gel loading buffers. Non-ionic polymer of saccharose.

Ficoll® 400 is a hydrophilic copolymer of saccharose and epichlorohydrin, that readily so ...
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CHF117.50/Pack Qty. 

excl. VAT. | 25 g per Pack Qty.

Art. No. CN90.2

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Ficoll® 400 for molecular biology and biochemistry

Ficoll® 400 is a hydrophilic copolymer of saccharose and epichlorohydrin, that readily solubilises in water and aqueous solutions. It may be solubilised at concentrations of up to 50 %, and is mainly used in the following applications: A) In hybridisation buffer acc. to Denhardt, Ficoll® 400 reduces non specific binding of labelled DNA to the filter membrane. B) In gel loading buffer it supports DNA application to agarose gels by increasing the density of the sample. C) In continuous or discontinuous density gradients, Ficoll® 400 is used as matrix for fractioning of cells (e.g. lymphocytes) or sub-cellular particles, also taking advantage of its ability to stabilise membrane-coupled components and the functional as well as the morphological integrity of cells. Autoclavable at neutral pH.

Ficoll® 400
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Order No. Pack Qty. Pack. Price Quantity
CN90.1 10 g plastic CHF55.90
CN90.2 25 g plastic CHF117.50
CN90.3 100 g plastic CHF377.30
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Guarantee analysis

Appearancewhite powder  
Solubility (10 % in H2O)clear, colourless  
Loss on drying≤3 %  

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