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5-Carboxytetramethylrhodamine-PEG3-Azide (5-TAMRA-PEG3-Azide), 1 mg

≥90 %
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5-((2-(2-(2-(2-Azidoethoxy)ethoxy)ethoxy)ethyl)carbamoyl)-2‑(6-(dimethylamino)-3‑(dimethyliminio)-3H‑xanthen-9‑yl)benzoate, 5-TAMRA-PEG3-Azide
Empirical formula C33H38N6O7
Molar mass (M) 630,69 g/mol
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. [1380486-02-1]

Fluorescent dye
For a 10 mM solution: add 158 µl to 1 mg.

Type analysis

CHF62.30/Pack Qty. 

excl. VAT. | 1 mg per Pack Qty.

Art. No. 7808.1

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Technical Information
Emissions maximum 579 nm  
5-Carboxytetramethylrhodamine-PEG3-Azide (5-TAMRA-PEG3-Azide)
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7808.1 1 mg plastic CHF62.30
7808.2 5 mg plastic CHF107.70
7808.3 10 mg plastic CHF164.50
7808.4 100 mg plastic CHF640.50
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General information

Click Chemistry

The copper(I)‑catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.


References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.


Tags

Fluorescent Tags
Carl ROTH offers a selection of fluorescent tags for labelling of (bio-)molecules via click chemistry. All available tags are suitable for fluorescence spectroscopy applications due to their excellent absorption properties and good solubility. Tags with PEG-spacer are beneficial for applications with as little interaction between the analyte and the tag as possible. The coumarine derivative (Art No. 7811) offers a unique feature: fluorescence is only detected after the coupling via click reaction.


Analysis certificates

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The following analysis certificates have been found:

Type analysis

Appearancedark red solid  
Assay (HPLC)≥90 %  
Absorption max. (DMF)546 nm  
Emission max. (DMF)579 nm  
Molar extinction coefficient ε91 000 cm -1M-1  
SolubilityDMSO, DMF, MeOH, Water (low)  

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