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ROTIPHORESE®10x TBE Buffer, 1 l, glass, 1 x 1 l

ROTIPHORESE® 10x conc.
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Danger
H360FD
i may damage fertility. May damage the unborn child
P201 P280 P308+P313
i obtain special instructions before use, wear protective gloves/eye protection, IF exposed or concerned: Get medical advice/attention
Pack Qty.
Pack.
Packaging
TBE-buffer
Density (D) 1,068 g/cm³
Boiling point (bp) >100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1

TBE-buffer for preparing sequencing gels and as running buffer in electrophoresis.
Concentrated TBE tends to precipitate salts during storage. Mix before use!

ROTIPHORESE®10x TBE buffer contains 1,0 M Tris-Borate pH 8,3 and 20 mM EDTA in distilled, deionised water; pH 8,3.

Suitable for manufacturing sequencing gels with ROTIPHORESE Gel 40 and as a running buffer.
Product details
Composition

CHF40.50/Pack Qty. 

excl. VAT. | 1 l per Pack Qty.

Art. No. 3061.1

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Product details



ROTIPHORESE®10x TBE Buffer ROTIPHORESE® 10x conc.

ROTIPHORESE®10x TBE buffer contains 1,0 M Tris-Borate pH 8,3 and 20 mM EDTA in distilled, deionised water; pH 8,3.

Suitable for manufacturing sequencing gels with ROTIPHORESE Gel 40 and as a running buffer.


Directions for use

ROTIPHORESE®TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.


Mechanism

TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.



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Order No. Pack Qty. Pack. Packaging Price Quantity
3061.1 1 l glass 1 x 1 l CHF40.50
3061.2 5 l plastic 1 x 5 l CHF132.90
3061.3 2.5 l glass 1 x 2.5 l CHF83.90
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Composition

1.0 M TRIS borate (pH 8.3), 20 mM EDTA., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).

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