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ROTIPHORESE®Sequencing gel buffer concentrate

ROTIPHORESE® 50 % (w/v, 8,3 M) in 10x TBE
Icon_ready-to-use Ready-to-use products and reagents
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Pack Qty.
Pack.
Sequencing gel solutions, Gel solution
Density (D) 1,185 g/cm³
Boiling point (bp) 100 °C
Storage temp. +4 to +15 °C
WGK 1

Solution of urea in 10x TBE for DNA sequencing
One liter is sufficient for 160 gels (30 x 40 cm)

Composition

CHF34.30/Pack Qty. 

excl. VAT. | 250 ml per Pack Qty.

Art. No. 3050.1

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Product details


  • For preparation of 4-20% sequencing gels
  • Most simple handling of three optimised ready-to-use solutions
  • No tedious dissolving of urea
  • Prevents acrylamide dusts
  • Reproducible, superior quality of gels
  • In tried-and-tested ROTIPHORESE® quality

Convenient set containing three ready-made solutions for easy and fast casting of sequencing gels. Solutions are produced in ROTIPHORESE® quality and are perfectly matched - providing superior, reproducible quality and high sequence resolution.
In addition, the ROTIPHORESE®Sequencing system allows flexible adaptation to the desired gel variant, so that the amount of urea can be varied in addition to the acrylamide concentration. By using the ROTIPHORESE®sequencing gel buffer concentrate and ROTIPHORESE®Sequencing gel diluent contained in the set, gels with 50 % urea content are obtained. By adding defined quantities of ROTIPHORESE®10x TBE buffer or water, the urea content can be reduced to up to 40 %. "


Directions for use

Handling is most simple: Measure and mix certain amounts of all three components, add APS and TEMED, and cast the gel. We recommend using the Carl ROTH sequencing electrophoresis chamber (order no. AE28).



ROTIPHORESE®Sequencing gel buffer concentrate ROTIPHORESE® 50 % (w/v, 8,3 M) in 10x TBE

Ready-to-use 10x TBE stock solution (1 M Tris borate, 20 mM EDTA buffer, pH 8,3) with 50 % urea .
For the preparation of polyacrylamide sequencing gels with 40-50 % urea (6,7-8,3 M).


Also part of the ROTIPHORESE®DNA sequencing system (Art. No. A431.1).



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General information

Safe
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.

Superior quality
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.


The right choice for each and every application
The large variety of different ROTIPHORESE® solutions provides you with the right product for each and every application. ROTIPHORESE® gel solution may be used both in protein and nucleic acid electrophoresis. ROTIPHORESE® ready-to-use solutions for manual sequencing facilitate the preparation of sequencing gels of a constant high quality - also suitable for automatic sequencers.


ROTIPHORESE®Gel 30 and Gel 40 are ready-to-use, gas-stabilised acrylamide solutions with different concentrations and with different ratios of acrylamide to bisacrylamide. ROTIPHORESE® ready-to-use solutions can be used directly.


Recommendation for Use of ROTIPHORESE® Ready-Made Gel Solutions


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Composition

1.0 M TRIS borate (pH 8.3), 20 mM EDTA., 50 % urea., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).