Transport temp. cooled
ADR 9 III
For 100 Assays.
CHF415.30/Pack Qty. Campaign price!
excl. VAT. | 1 kit per Pack Qty.
Art. No. 7776.1
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- Highly reliable
- Easy to perform
- Fast detection procedure (only 30 min)
- Mild conditions (no DNA denaturation required)
- Modular system due to click chemistry
- Compatible with various dyes and readouts
- Compatible with multiplexing
- No cytotoxicity up to 1 mM EdU
The detection of cell proliferation is of utmost importance for monitoring of cell vitality, determining genotoxicity or evaluating anticancer drugs. Carl ROTH's cell proliferation assays based on EdU (5-ethynyl-2'-deoxyuridine) provide a superior alternative to BrdU (bromodeoxyuridine) assays.
Carl ROTH's EdU Click assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU Click assay utilizes click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol both reduces the total number of steps and significantly decreases the total amount of time.
The number at the end of the product name specifies the wavelength at which the included fluorescent marker is optimally excited.
S. Vidak et al., Proliferation of progeria cells is enhanced by lamina-associated polypeptide 2α (LAP2α) through expression of extracellular matrix proteins, Genes & Development 2015, 29, 2022-2036.
Additional costs resulting from such, will be invoiced (further information on request).
Please note: In order to guarantee optimum product quality, the dispatch of refrigerated transport products will be carried out on Mon and Tue only outside Germany!
Slight delays in delivery are therefore possible.
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• Fluorescence microscopy (ROTI®Kits for Imaging)
• Flow Cytometry (ROTI®Kits for Flow Cytometry)
• High Throughput Screening (ROTI®Kits for High Throughput Screening)
|Code in product name||Markers||Absorption (nm)||Emission (nm)||Filter configuration|
|-594||5/6 Sulforhodamine||584||603||Texas Red®|
|-647||Eterneon red (analogous to cyanine 5 azide)||643||662||Cy5T™|
|5/6-Sulforhodamine-101-PEG3-Azide (10 mM in DMSO)||130 µl|
|Buffer Additive||4 x 200 mg|
|DMSO||2 x 2 ml|
|Catalyst Solution||2 x 2 ml|
|Reaction Buffer||4 x 2 ml|
|Store at -20 °C (EdU, 5/6-Sulforhodamine-101-PEG3-Azide, Buffer Additive); +4 °C (DMSO, Catalyst Solution, Reaction Buffer)|