EdU Click FC-488
Transport temp. cooled
ADR 9 III
For 50 assays.
excl. VAT. | 1 kit per Pack Qty.
Art. No. 7779.1
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- Highly reliable
- Easy to perform
- Fast detection procedure (only 30 min)
- Mild conditions (no DNA denaturation required)
- Modular system due to click chemistry
- Compatible with various dyes and readouts
- Compatible with multiplexing
- No cytotoxicity up to 1 mM EdU
The ROTI®kits for Flow Cytometry allow the measurement of DNA synthesis in flow cytometry. Similar to BrdU assays, our assays utilise a uracil derivative (in this case ethynyldeoxyuridine, EdU) which is incorporated into the replicating DNA. EdU is not detected via antibodies; instead fluorescent dyes are coupled directly via click reaction. This fast and elegant approach allows for direct labelling of replicating DNA with the desired fluorescent dye. Thus, the assay is perfectly adaptable to the present facilities. The protocol includes only few steps and decreases the total amount of time.
A: negative control, cells without EdU incorporation.
B: non-proliferating cells without EdU incorporation (blue coloured left peak) and proliferating cells (S-phase) having incorporated EdU and are labeled with 6-FAM Azide (green coloured right peak).
Density blots of PI (propidium iodide) stained samples without (C) and with (D) EdU incubation and followed click reaction. The y-axis presents the FL1-Fluorescence intensity and the x-axis the content of DNA measured with FL3-area. Cell cycle phases are indicated as G1, S and G2/M phase.
The number at the end of the product name specifies the wavelength at which the included fluorescent marker is optimally excited.
1 N. Dinh Van et al., Modulation of HCV reinfection after orthotopic liver transplantation by fibroblast growth factor-2 and other non-interferon mediators. Gut 2016, 65, 1015-1023.
2 L. Pisarsky et al., Targeting Metabolic Symbiosis to Overcome Resistance to Anti-angiogenic Therapy. Cell Reports 2016, 15, 1-14.
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• Fluorescence microscopy (ROTI®Kits for Imaging)
• Flow Cytometry (ROTI®Kits for Flow Cytometry)
• High Throughput Screening (ROTI®Kits for High Throughput Screening)
|Code in product name||Markers||Absorption (nm)||Emission (nm)||Filter configuration|
|-594||5/6 Sulforhodamine||584||603||Texas Red®|
|-647||Eterneon red (analogous to cyanine 5 azide)||643||662||Cy5T™|
|6-FAM-Azide (10 mM in DMSO)||130 µl|
|Fixative Solution||5 ml|
|Saponin Reagent||50 ml|
|Catalyst Solution||2 ml|
|Buffer Additive||400 mg|