ROTI®Fect Plus, 1 ml
Approx. 500 assays per ml in 24wells. Dependent on the cell line used.
CHF359.20/Pack Qty. Campaign price!
excl. VAT. | 1 ml per Pack Qty.
Art. No. CL21.2
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ROTI®Fect Plus CELLPURE® for transfection
Highly sophisticated transfection reagent for liposoma mediated transfection of eukaryotic cell lines and primary cells in culture. ROTI®Fect PLUS further reduces the toxic effects of transfection, making it a versatile tool for transfection of sensitive cell lines or primary cells.
Based on this, ROTI®Fect PLUS is also especially recommended for transfection prior to in vivo analyses (siRNA-Assays, in vivo life microscopy, etc.), since the transfection stress is minimized, reducing stress-dependent effects like the rate of apoptosis-induced cells. Additionally, the stability of nucleic acid/lipid complexes is diminished in comparison with ROTI®Fect, therefore improving the release of nucleic acids in the cell.
Thus, the newly established liposoma formulation of ROTI®Fect PLUS guarantees gentle and highly efficient transfections, even when hard-to-transfect or particularly sensitive cell lines are handled.
- Particularly low cell toxicity
- Superior transfection efficiency with all cell lines
- Especially recommended for hard-to-transfect cell lines
- Particularly suited for use with primary cells
- Excellent reproducibility
- No serum inhibition
siRNA: 1BR3, HEK293, HeLa, LN-308, T98G. U87MG, UACC-257
Primary cells: 1BR3, 8VM, CGN, HMEC, HUVEC, PBMC, S15VM
Cell lines: 1BRneo, 143B, 293T, NSCLC, alphaTC1, B16-F10, BHK-21, C2C12, C6, CHO, CHO-K1, Colo205, COS-7, EVLC2, HaCaT, HAEC, HCT116, HEK293, HeLa, HepG2, Hep 3B, IMR32, Jurkat, Kelly, KG1, La1-5S, LN-308, M2-10B4, MDCK, MEF, MG-63, MIN6, MLEC32, mPAC, mpkCCD, N18TG2, N2a, N-Tera2, NG NIH-3T3, Panc-1, PC 12, Phi-NX, Raji, RAW264.7, RD, RF24, RGE, S2, S91, SMA-560, SK-N-BE, SW480, T98G, U-2 OS, Vero, et al.
Via ROTI®Fect PLUS transfected cells.A: Fluorescent light (520 nm), B: Standard light.
Above: COS7 cells (0.5 × 105 cells / cm2 in 24-well).
Transfection after 24 h using 0.9 μg pCMV-GFP + 3.6 μl ROTI®Fect PLUS (ratio 1:4). Analysis after 48 h.
Below: BHK cells (0.2 × 105 cells / cm2 in 24-well).
Transfection after 24 h using 0.3 μg pCMV-GFP + 1.2 μl ROTI®Fect PLUS (ratio 1:4). Analysis after 48 h.
Transfection efficiency subject to ratio of DNA to ROTI®Fect PLUS. For most cell lines, the optimised composition of transfection complex is 1:3 to 1:4.
Vero: 1.2 × 105 cells / cm2; MDCK: 1.2 × 105 cells / cm2; COS7: 5 × 104 cells / cm2; HeLa: 1 × 105 cells / cm2. Transfection with pCMV-LacZ 24 h after sawing in 48 wells in serum-free DMEM. Analysis (ONPG assay) after 48 h.
Transfection efficiency of ROTI®Fect PLUS given in percentage. For standardisation, transfection efficiency of ROTI®Fect was set as reference (100 %).
Compared to ROTI®Fect, transfection efficiency achieved by ROTI®Fect PLUS is at least equivalent. For some cell lines known to be hard-to-transfect with ROTI®Fect, e.g. Vero, CV1, or CHO-K1 cells, ROTI®Fect PLUS achieves multiple enhancement of transfection efficiency.
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