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ROTI®Fect Plus, 1 ml

CELLPURE® for transfection
Icon_ready-to-use
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Transfection reagents
Storage temp. +4 °C
WGK 3

Reagent for transfection of all eukaryotic cells in culture, particularly suited for hard-to-transfect cell lines.
Approx. 500 assays per ml in 24wells. Dependent on the cell line used.

Highly sophisticated transfection reagent for liposoma mediated transfection of eukaryotic cell line ...
Product details
CHF460.60 /Pack Qty.

CHF359.20/Pack Qty.  Campaign price!

excl. VAT. | 1 ml per Pack Qty.

Art. No. CL21.2

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ROTI®Fect Plus CELLPURE® for transfection

Highly sophisticated transfection reagent for liposoma mediated transfection of eukaryotic cell lines and primary cells in culture. ROTI®Fect PLUS further reduces the toxic effects of transfection, making it a versatile tool for transfection of sensitive cell lines or primary cells.
Based on this, ROTI®Fect PLUS is also especially recommended for transfection prior to in vivo analyses (siRNA-Assays, in vivo life microscopy, etc.), since the transfection stress is minimized, reducing stress-dependent effects like the rate of apoptosis-induced cells. Additionally, the stability of nucleic acid/lipid complexes is diminished in comparison with ROTI®Fect, therefore improving the release of nucleic acids in the cell.
Thus, the newly established liposoma formulation of ROTI®Fect PLUS guarantees gentle and highly efficient transfections, even when hard-to-transfect or particularly sensitive cell lines are handled.

  • Particularly low cell toxicity
  • Superior transfection efficiency with all cell lines
  • Especially recommended for hard-to-transfect cell lines
  • Particularly suited for use with primary cells
  • Excellent reproducibility
  • No serum inhibition
CL21_RotiFectPLUS_Ablaufschema

Successfully tested on the following cell types

siRNA: 1BR3, HEK293, HeLa, LN-308, T98G. U87MG, UACC-257
Primary cells: 1BR3, 8VM, CGN, HMEC, HUVEC, PBMC, S15VM
Cell lines: 1BRneo, 143B, 293T, NSCLC, alphaTC1, B16-F10, BHK-21, C2C12, C6, CHO, CHO-K1, Colo205, COS-7, EVLC2, HaCaT, HAEC, HCT116, HEK293, HeLa, HepG2, Hep 3B, IMR32, Jurkat, Kelly, KG1, La1-5S, LN-308, M2-10B4, MDCK, MEF, MG-63, MIN6, MLEC32, mPAC, mpkCCD, N18TG2, N2a, N-Tera2, NG NIH-3T3, Panc-1, PC 12, Phi-NX, Raji, RAW264.7, RD, RF24, RGE, S2, S91, SMA-560, SK-N-BE, SW480, T98G, U-2 OS, Vero, et al.


CL21_Zellen
Figure:
Via ROTI®Fect PLUS transfected cells.A: Fluorescent light (520 nm), B: Standard light.
Above:
COS7 cells (0.5 × 105 cells / cm2 in 24-well).
Transfection after 24 h using 0.9 μg pCMV-GFP + 3.6 μl ROTI®Fect PLUS (ratio 1:4). Analysis after 48 h.
Below:
BHK cells (0.2 × 105 cells / cm2 in 24-well).
Transfection after 24 h using 0.3 μg pCMV-GFP + 1.2 μl ROTI®Fect PLUS (ratio 1:4). Analysis after 48 h.
CL21_Diagramm_02
Figure:
Transfection efficiency subject to ratio of DNA to ROTI®Fect PLUS. For most cell lines, the optimised composition of transfection complex is 1:3 to 1:4.
Vero: 1.2 × 105 cells / cm2; MDCK: 1.2 × 105 cells / cm2; COS7: 5 × 104 cells / cm2; HeLa: 1 × 105 cells / cm2. Transfection with pCMV-LacZ 24 h after sawing in 48 wells in serum-free DMEM. Analysis (ONPG assay) after 48 h.
CL21_Diagramm
Figure:
Transfection efficiency of ROTI®Fect PLUS given in percentage. For standardisation, transfection efficiency of ROTI®Fect was set as reference (100 %).
Compared to ROTI®Fect, transfection efficiency achieved by ROTI®Fect PLUS is at least equivalent. For some cell lines known to be hard-to-transfect with ROTI®Fect, e.g. Vero, CV1, or CHO-K1 cells, ROTI®Fect PLUS achieves multiple enhancement of transfection efficiency.


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