ROTI®GelStain, 5 ml, 5 x 1 ml
Ready-to-use products and reagents
Boiling point (bp) 100 °C
Storage temp. +4 °C
WGK 2
1 ml is sufficient for staining of approx. 600 minigels with 30 ml agarose volume each.
CHF572.60/Pack Qty.
excl. VAT. | 5 ml per Pack Qty.
Art. No. 3865.2
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Product details
Non-toxic and non-carcinogenic dyes for fluorescent staining of nucleic acids in agarose gels. Highly sensitive and applied just like ethidium bromide.
The ROTI®GelStain dyes are used as additives to the gel solution and the running buffer,partly also post-run staining is possible Both ROTI®GelStain dyes can be excited by UV light, additionally, ROTI®GelStain Red may be excited by blue light. Resulting signals may be detected by ethidium bromide broadband photo filters.
ROTI®GelStain ready-to-use, for electrophoresis
ROTI®GelStain is a versatile staining reagent for detection of all nucleic acids in agarose gels.
Suitable for staining of dsDNA, ssDNA and RNA.
- Green fluorescence
- Alternative for ethidium bromide, non-toxic, non-mutagen
- Nearly as sensitive as ethidium bromide, up to 1,5 ng/mm2 nucleic acid
- For use with common broad-band ethidium bromide photo filters
- Excitation via UV light (310 nm)
- Compatible with all usual down-stream applications
Amplicons of a mouse genotyping (left), and restriction digested DNA (right), respectively, separated in 2,5 and 1 % agarose ROTI®garose NEEO with ROTI®GelStain (5 µl / 100 ml agarose). Pictures were taken using standard ethidium bromide photo filters.
With kind permission of Prof. R. Slany, Dept. of Genetics, Friedrich Alexander University, Erlangen.
The dye may be used exactly in the same way as ethidium bromide - for staining in-gel, staining through running buffer, or post-run staining -, and may replace this toxic and potentially mutagen reagent completely.
ROTI®-GelStain detects up to 1,5 ng/mm2 nucleic acid, which is approx. 0,2 ng per band, and is, therefore, nearly as sensitive as ethidium bromide.
When bound to nucleic acids in agarose gels, ROTI®-GelStain emits a brightly green fluorescence that may be documented by all usual broadband ethidium bromide or SYBR® Green foto filters.
ROTI®-GelStain is compatible with all standard down-stream applications and has been successfully tested for use with gel extraction, ligation, transformation and transfection.
Excitation maximum (bound to DNA): 290-320 nm
Emission maximum (bound to DNA): 515 nm
| Max. excitation (DNA-bound) | 290-320 nm |
| Emissions max. (DNA-bound) | 515 nm |
| Colour | fluorescent/green |
| Sensitivity | 0,2 ng/band |
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| Order No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
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| 3865.1 | 1 ml | plastic | 1 x 1 ml | CHF129.50 |
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| 3865.2 | 5 ml | plastic | 5 x 1 ml | CHF572.60 |
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