SYBR® Green DNA dye, 1.8 ml, 1 x 1.8 ml
Ready-to-use products and reagents
Transport temp. ambient temp.
WGK 3
1.8 ml are sufficient for gel run and staining of approx. 3,600 lanes (5 µl sample + 0.5 µl dye).
SYBR® Green I bounds to the minor groove of DNA and is therefore not intercalating. Upon excitation it emits a bright green fluorescence. The sensitivity is up to 0.01 ng per band and is therefore 20 times higher than ethidium bromide.
SYBR® Green I has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects.
CHF21.70/Pack Qty. Campaign price!
excl. VAT. | 1.8 ml per Pack Qty.
Art. No. 1CN2.1
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Product details
This is the DNA dye SYBR® Green I, a staining reagent for detection of double stranded nucleic acid in agarose and polyacrylamide gels. The dye is added directly to the DNA/Loading buffer mixture and then loaded onto the gel.
SYBR® Green I bounds to the minor groove of DNA and is therefore not intercalating. Upon excitation it emits a bright green fluorescence. The sensitivity is up to 0.01 ng per band and is therefore 20 times higher than ethidium bromide.
SYBR® Green I has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects.
- Direct addition to DNA sample
- For excitation with UV light and blue light
- Non-toxic alternative for ethidium bromide
SYBR® Green DNA dye can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE®PROfessional runVIEW, Art. No. 4849.1). The gels may be documented by all usual broadband ethidium bromide- or SYBR® Green foto filters.
Excitation maximum (bound to DNA): approx. 254 nm and 495 nm
Emission maximum (bound to DNA): 521 nm
SYBR® Green DNA dye 11x conc., for electrophoresis
| Max. excitation (DNA-bound) |
254 nm 495 nm |
| Amount of traces | ca. 2000/ml |
| Emissions max. (DNA-bound) | 521 nm |
| Sensitivity | 0,01 ng/band |
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| Order No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 1CN2.1 | 1.8 ml | plastic | 1 x 1.8 ml | CHF21.70 |
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| 1CN2.2 | 9 ml | plastic | 5 x 1.8 ml | CHF85.05 |
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In stock
Available at short notice
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- Subtotal: 0.00
Downloads
Certificates of Analysis
Type analysis
| Appearance | orange solution |
| Each batch is functionally tested for its suitability in electrophoresis. | |