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ROTI®Blue, 1 l

5x conc.
Icon_ready-to-use Ready-to-use products and reagents
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Warning
H290
i may be corrosive to metals
P234 P390 P406
i keep only in original packaging, absorb spillage to prevent material damage, store in a corrosion-resistant container with a resistant inner liner
Pack Qty.
Pack.
Coomassie®-Staining solution
Density (D) 1,24 g/cm³
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 8 III
WGK 1
UN-Nr. 1805

Colloidal Coomassie® staining solution for protein gels.
1 l concentrated solution is sufficient for approx. 50 mini gels.

ROTI®Blue is a colloidal Coomassie® brilliant blue G250 staining agent for proteins in polyacrylamid gels. Its colloidal features enable the staining agent to be bound to the proteins with high specificity and only minimally to the gel matrix. There is no longer any need for the extensive washing steps for background decoloring.
Product details
Type analysis

CHF129.50/Pack Qty. 

excl. VAT. | 1 l per Pack Qty.

Art. No. A152.1

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from 6 Pack Qty. CHF123.02/Pack Qty.
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Product details



ROTI®Blue 5x conc.

ROTI®Blue is a colloidal Coomassie® brilliant blue G250 staining agent for proteins in polyacrylamid gels. Its colloidal features enable the staining agent to be bound to the proteins with high specificity and only minimally to the gel matrix. There is no longer any need for the extensive washing steps for background decoloring.

  • Most simple application
  • No destaining necessary
  • 5x more sensitive than Coomassie® R250
A152_Banden
Figures:
A: Horizontal IEF for determination of wheat-varieties.
With kind permission of R. Knoblauch, LTZ Referat 23, Karlsruhe
B: SDS-PAGE ROTI®Mark Standard.

ROTI®Blue is approximately 5 x more sensitive than standard Coomassie® staining solutions. Staining time takes 2 to 12 hours depending on the desired band intensity.
Due to the fact that proteins may be stained without fixing the gel, membrane transfer and gel elution are still possible following staining.
Particularly well suited for staining prior to MS-analyses (e. g. MALDI) and prior to gel elution.



Technical Information
Sensitivity 10 
Use Coomassie® staining solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible. 
ROTI®Blue
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Order No. Pack Qty. Pack. Price Quantity
A152.1 1 l plastic CHF129.50
A152.2 250 ml plastic CHF44.70
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The following analysis certificates have been found:

Type analysis

Appearancedark blue, colloidale solution
pH-value1 - 4