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3-Azido-7-hydroxycoumarin, 1 mg

From  baseclick
≥98 %
Icon_Baseclick_Logo-CMYK
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Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H302-H315-H319-H335
i harmful if swallowed, causes skin irritation, causes serious eye irritation, may cause respiratory irritation
P261 P280 P302+P352 P304+P340 P305+P351+P338
i avoid breathing dust, wear protective gloves/protective clothing/eye protection/face protection, IF ON SKIN: Wash with plenty of water, IF INHALED: Remove person to fresh air and keep comfortable for breathing, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
3-Azido-7-hydroxy-chromen-2-one
Empirical formula C9H5N3O3
Molar mass (M) 203,15 g/mol
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. 817638-68-9

Switch-on dye to label DNA/RNA via click chemistry
3-Azido-7-hydroxycoumarin has the special property to become fluorescent only when it is click ligated. The formed triazole expands the electron system of the dye and enables fluorescence. Therefore, there is no background signal of the click unligated leftover dye. This dye is a valuable tool for nucleic acid labeling.

€53.75/Pack Qty. 

excl. VAT. | 1 mg per Pack Qty.

Art. No. 7811.1

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from 6 Pack Qty. €51.06/Pack Qty.
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Product details



3-Azido-7-hydroxycoumarin ≥98 %

Technical Information
Emissions maximum 480 nm (after click reaction) 
Instructions for use, addition For a 10 mM solution: add 492 µl to 1 mg. 
3-Azido-7-hydroxycoumarin
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Art. No. Pack Qty. Pack. Price Quantity
7811.1 1 mg plastic

€53.75

7811.2 5 mg plastic

€145.15

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Downloads / MSDS

General information

Click Chemistry

The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.


References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.


Click Chemistry

In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.


Tags

Fluorescent Tags

Carl ROTH offers a selection of fluorescent tags for labelling of (bio-)molecules via click chemistry. All available tags are suitable for fluorescence spectroscopy applications due to their excellent absorption properties and good solubility. Tags with PEG-spacer are beneficial for applications with as little interaction between the analyte and the tag as possible. The coumarine derivative (Art No. 7811) offers a unique feature: fluorescence is only detected after the coupling via click reaction.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearanceamber to brown crystalline solid
Assay (HPLC)≥98 %