ROTI®-MagBeads COOH, 2 ml
excl. VAT. | 2 ml per Pack Qty.
Art. No. HP58.1
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ROTI®-MagBeads COOH 10 mg/ml, for biochemistry and molecular biology
- Easy and standardized handling
- Fast and easy batch format without columns
- Allows harsh pH values and high ionic strength
- For ligand coupling prior to purification of biomolecules, immunoassays, and cell sorting
Roti®-MagBeads COOH is a matrix for simple and efficient coupling of any chosen ligand that can be coupled to carboxylic groups, such as antibo-dies, proteins and oligonucleotides. Subsequently, the coated beads can be used for isolation of proteins and peptides, nucleic acids and other bio-molecules, and for positive or negative cell sorting. In case antibodies are coupled to the beads, immunoassays can be carried out.
The magnetic properties enable easy and quick washing steps, allowing one final bioassay to take place in approx. 10 mins. The small bead size of 1 µm in average provides a large surface with excellent coupling capacities. Handling is easy and identical to standardized protocols for beads of other suppliers, providing easy establishment of the assays. The high ligand coupling capacity combined with low non-specific binding minimizes the signal-to-noise ratio. Subsequent assays are well reproducible with high recovery rates.
The Matrix of Roti®-MagBeads COOH consists of uniform, superpara-magnetic silica beads coated with activated surface (-COOH). In those beads, nanometer-sized particles of magnetic iron(II/III)oxide are embed-ded in silicium oxide, resulting in high magnetic mobility with low aggre-gation potential. The surface is made from pure activated silicium oxide and allows application of harsh pH values as well as of media with high ionic strength.
Well suited for automated and manual applications.
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|Order No.||Pack Qty.||Pack.||Price||Quantity|
Available at short notice
Delivery time on request
|Concentration||10 ±0,5 mg/ml|
|Size distribution||0,7-1,4 µm|
|Mean particle sizing (CI90 %)||1,0 ±0,05 µm|
|Bead number||6-12 x 109 Beads/ml|
|Magnetic iron content||≥50 %|
|Suspension in PBS (pH 7,4); 0,05 % sodium azide|