Technical Data Sheet
ROTI®GelStain Red, 5 ml, 5 x 1 ml
Ready-to-use products and reagents
Storage temp. +4 °C
WGK 2
Red fluorescent staining reagent for non-toxic staining of nucleic acids in agarose and polyacrylamide gels.
Art. No. 0984.2
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Product details
Non-toxic and non-carcinogenic dyes for fluorescent staining of nucleic acids in agarose gels and polyacrylamide gels. Highly sensitive and applied just like ethidium bromide.
The ROTI®GelStain dyes are used as additives to the gel solution and the running buffer, partly also post-run staining is possible. Both ROTI®GelStain dyes can be excited by UV light and by blue light. Resulting signals may be detected by ethidium bromide broadband photo filters.
ROTI®GelStain Red 20 000x conc., ready-to-use
ROTI®-GelStain Red is a versatile staining reagent for detection of all nucleic acids in agarose and polyacrylamide gels.
Suitable for staining of dsDNA, ssDNA and RNA.
- Red fluorescence
- Alternative to ethidium bromide, non-toxic, non-mutagen
- Detection of > 0.3 ng nucleic acid per band
- Usuable with the same filters as ethidium bromide
- Excitation via UV light (310 nm) and blue light (540 nm)
- Compatible with all usual down-stream applications
ROTI®-GelStain Red is a red fluorescent dye that can be used like ethidium bromide for staining in gel (addition to gel solution). Due to the low membrane permeability of the dye ROTI®-GelStain Red is non-mutagenic and non-toxic. It is suitable for staining of dsDNA, ssDNA and RNA.
ROTI®-GelStain detects up to 0.3 ng per band, making it more sensitive than ethidium bromide.
Bound to nucleic acid it emits a brightly red fluorescence that can be documented by all usual ethidium bromide foto filters. ROTI®-GelStain Red is compatible with all usual down-stream applications.
Excitation maximum (bound to DNA): approx.310 nm and 540 nm
Emission maximum (bound to DNA): 630 nm
| Colour | fluorescent/red |
| Sensitivity | 0,3 ng/band |
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
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| 0984.2 | 5 ml | plastic | 5 x 1 ml | NA |
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| 0984.1 | 1 ml | plastic | 1 x 1 ml | NA |
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Downloads / MSDS
General information
Summary Staining of Nucleic Acid in Gels
| Product | Sensitivity | Description | Excitation |
| Ethidium bromide solution | 0.5-5 ng/band | Standard fluorescent staining | 254-330 nm, 500 nm UV- and blue light |
| SYBR® Green DNA dye | 0.01 ng/band | Non-toxic, green fluorescent staining | 254 nm and 495 nm UV- and blue light |
| ROTI®GelStain | 0.3 ng/band | Non-toxic, green fluorescent staining | 302 nm and 490 nm UV- and blue light |
| ROTI®GelStain Red | 0.3 ng/band | Non-toxic, red fluorescent staining | 310 nm, 540 nm UV- and blue light |
| ROTI®Methylene blue staining concentrate | 10 ng/band | Reversible, non-toxic blue staining | White light |
| ROTI®Black N | <0.1 ng/band | Silver staining of DNA polyacrylic amide gels | White light |
Certificates of Analysis
Type analysis
| Appearance | red, transparent liquid, no precipitation |
| Staining of DNA in agarose gels | complies |
| Staining of DNA in PA gels | complies |