Technical Data Sheet
ROTI®GelStain, 1 ml, 1 x 1 ml
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
1 ml is sufficient for staining of approx. 600 minigels with 30 ml agarose volume each.
excl. VAT. | 1 ml per Pack Qty.
Art. No. 3865.1
Fits to / Accessories
Non-toxic and non-carcinogenic dyes for fluorescent staining of nucleic acids in agarose gels and polyacrylamide gels. Highly sensitive and applied just like ethidium bromide.
The ROTI®GelStain dyes are used as additives to the gel solution and the running buffer, partly also post-run staining is possible. Both ROTI®GelStain dyes can be excited by UV light and by blue light. Resulting signals may be detected by ethidium bromide broadband photo filters.
ROTI®GelStain 20 000x conc., ready-to-use
ROTI®GelStain is a versatile staining reagent for detection of all nucleic acids in agarose and polyacrylamide gels.
- Green fluorescence
- Alternative for ethidium bromide, non-toxic and non-mutagenic
- For dsDNA, ssDNA and RNA
- Sensitivity comparable to ethidium bromide (approx. 0.3 ng / band)
- For use with common broad-band ethidium bromide photo filters
- Excitation via UV light (302 nm) and blue light (490 nm)
- Compatible with all usual down-stream applications
The dye can be used in a similar manner as ethidium bromide. By adding the dye to the liquid gel, the nucleic acids are stained during the gel run.
ROTI®GelStain has a sensitivity of 0.2 ng/band and is thus even slightly more sensitive than ethidium bromide.
When bound to nucleic acids in agarose gels, ROTI®GelStain emits a brightly green fluorescence that may be documented by all usual broadband ethidium bromide or SYBR® Green foto filters.
ROTI®-GelStain is compatible with all standard down-stream applications and has been successfully tested for use with gel extraction, ligation, transformation and transfection.
Amplicons of a mouse genotyping (left), and restriction digested DNA (right), respectively, separated in 2,5 and 1 % agarose ROTI®Garose NEEO with ROTI®GelStain (5 µl / 100 ml agarose). Pictures were taken using standard ethidium bromide photo filters.
With kind permission of Prof. R. Slany, Dept. of Genetics, Friedrich Alexander University, Erlangen.
Excitation maximum (bound to DNA): 302 nm (UV) and 490 nm (blue light)
Emission maximum (bound to DNA): 520 nm
|Max. excitation (DNA-bound)||302 nm (UV), 490 nm|
|Emissions max. (DNA-bound)||520 nm|
- Subtotal: 0.00
|Art. No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|3865.1||1 ml||plastic||1 x 1 ml||
|3865.2||5 ml||plastic||5 x 1 ml||
No longer available
Delivery date currently unknown
Summary Staining of Nucleic Acid in Gels
|Ethidium bromide solution||0.5-5 ng/band||Standard fluorescent staining||254-330 nm, 500 nm UV- and blue light|
|SYBR® Green DNA dye||0.01 ng/band||Non-toxic, green fluorescent staining||254 nm and 495 nm UV- and blue light|
|ROTI®GelStain||0.3 ng/band||Non-toxic, green fluorescent staining||302 nm and 490 nm UV- and blue light|
|ROTI®GelStain Red||0.3 ng/band||Non-toxic, red fluorescent staining||310 nm, 540 nm UV- and blue light|
|ROTI®Methylene blue staining concentrate||10 ng/band||Reversible, non-toxic blue staining||White light|
|ROTI®Black N||<0.1 ng/band||Silver staining of DNA polyacrylic amide gels||White light|