Technical Data Sheet
ROTI®GelStain, 1 ml, 1 x 1 ml
Products with optimised manufacturing process in terms of sustainability.
Ready-to-use products and reagents
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
WGK 2
For non-toxic fluorescent staining of nucleic acids in agarose gels. Green fluorescence. Direct addition to the agarose solution.
€127.95/Pack Qty.
excl. VAT. | 1 ml per Pack Qty.
Art. No. 3865.1
Fits to / Accessories
Product details
Non-toxic and non-carcinogenic dyes for fluorescent staining of nucleic acids in agarose gels and polyacrylamide gels. Highly sensitive and applied just like ethidium bromide.
The ROTI®GelStain dyes are used as additives to the gel solution and the running buffer, partly also post-run staining is possible. Both ROTI®GelStain dyes can be excited by UV light and by blue light. Resulting signals may be detected by ethidium bromide broadband photo filters.
ROTI®GelStain 20 000x conc., ready-to-use
ROTI®GelStain is a versatile staining reagent for detection of all nucleic acids in agarose and polyacrylamide gels.
- Green fluorescence
- Alternative for ethidium bromide, non-toxic and non-mutagenic
- For dsDNA, ssDNA and RNA
- Sensitivity comparable to ethidium bromide (approx. 0.3 ng / band)
- For use with common broad-band ethidium bromide photo filters
- Excitation via UV light (302 nm) and blue light (490 nm)
- Compatible with all usual down-stream applications
The dye can be used in a similar manner as ethidium bromide. By adding the dye to the liquid gel, the nucleic acids are stained during the gel run.
ROTI®GelStain has a sensitivity of 0.2 ng/band and is thus even slightly more sensitive than ethidium bromide.
When bound to nucleic acids in agarose gels, ROTI®GelStain emits a brightly green fluorescence that may be documented by all usual broadband ethidium bromide or SYBR® Green foto filters.
ROTI®-GelStain is compatible with all standard down-stream applications and has been successfully tested for use with gel extraction, ligation, transformation and transfection.
Excitation maximum (bound to DNA): 302 nm (UV) and 490 nm (blue light)
Emission maximum (bound to DNA): 520 nm
Amplicons of a mouse genotyping (left), and restriction digested DNA (right), respectively, separated in 2,5 and 1 % agarose ROTI®garose NEEO with ROTI®GelStain (5 µl / 100 ml agarose). Pictures were taken using standard ethidium bromide photo filters.
With kind permission of Prof. R. Slany, Dept. of Genetics, Friedrich Alexander University, Erlangen.
| Max. excitation (DNA-bound) | 302 nm (UV), 490 nm |
| Emissions max. (DNA-bound) | 520 nm |
| Colour | fluorescent/green |
| Sensitivity | 0,2 ng/band |
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
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| 3865.1 | 1 ml | plastic | 1 x 1 ml |
€127.95 |
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| 3865.2 | 5 ml | plastic | 5 x 1 ml |
€568.70 |
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In stock
Available
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No longer available
Delivery date currently unknown
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Downloads / MSDS
General information
Summary Staining of Nucleic Acid in Gels
| Product | Sensitivity | Description | Excitation |
| Ethidium bromide solution | 0.5-5 ng/band | Standard fluorescent staining | 254-330 nm, 500 nm UV- and blue light |
| SYBR® Green DNA dye | 0.01 ng/band | Non-toxic, green fluorescent staining | 254 nm and 495 nm UV- and blue light |
| ROTI®GelStain | 0.3 ng/band | Non-toxic, green fluorescent staining | 302 nm and 490 nm UV- and blue light |
| ROTI®GelStain Red | 0.3 ng/band | Non-toxic, red fluorescent staining | 310 nm, 540 nm UV- and blue light |
| ROTI®Methylene blue staining concentrate | 10 ng/band | Reversible, non-toxic blue staining | White light |
| ROTI®Black N | <0.1 ng/band | Silver staining of DNA polyacrylic amide gels | White light |