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ROTI®Methylene blue staining concentrate, 3 ml

200x conc.
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Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P305+P351+P338
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking, wear protective gloves/protective clothing/eye protection/face protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Methylene blue staining concentrate
Density (D) ~1 g/cm³
Boiling point (bp) ~100 °C
Flash point (flp) ~40 °C
Storage temp. +4 °C
ADR 3 III
WGK 1
UN-Nr. 1170

For reversible staining of DNA in agarose gels
12 ml Roti®-methylene blue staining concentrate is sufficient for more than 50 mini gels.

ROTI®Methylene blue staining concentrate was specially developed for sensitive staining of DNA in agarose gels. Sensitivity reaches up to 10 ng per band. Staining can be conducted in a simple staining bowl, a transilluminator is not required.
Product details

€51.10/Pack Qty. 

excl. VAT. | 3 ml per Pack Qty.

Art. No. 0648.1

In stock
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Product details



ROTI®Methylene blue staining concentrate 200x conc.

ROTI®Methylene blue staining concentrate was specially developed for sensitive staining of DNA in agarose gels. Sensitivity reaches up to 10 ng per band. Staining can be conducted in a simple staining bowl, a transilluminator is not required.

  • Highly reversible alternative to ethidium bromide
  • High sensivity (in minimum 10 ng/band)
  • No transilluminator required
  • Solution can be used repeatedly
  • Sufficient for more than 50 mini gels

Mechanism

ROTI®Methylene blue staining concentrate binds to the negativly charged DNA backbone by salt bridges formed through quaternary ammonia complexes. The staining is very easy to remove by washing or replacement by salts.


Directions for use

For staining DNA agarose gels, DNA polyacrylamide gels, for reversible staining of Southern- and Northern-Blots.
Staining can be performed in a simple staining dish, a transilluminator is not required. The diluted staining solution can be used several times.



ROTI®Methylene blue staining concentrate
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0648.1 3 ml plastic

€51.10

0648.2 12 ml plastic

€145.15

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Downloads / MSDS


General information

Summary Staining of Nucleic Acid in Gels

Product Sensitivity Description Excitation
Ethidium bromide solution 0.5-5 ng/band Standard fluorescent staining 254-330 nm, 500 nm UV- and blue light
SYBR® Green DNA dye 0.01 ng/band Non-toxic, green fluorescent staining 254 nm and 495 nm UV- and blue light
ROTI®GelStain 0.3 ng/band Non-toxic, green fluorescent staining 302 nm and 490 nm UV- and blue light
ROTI®GelStain Red 0.3 ng/band Non-toxic, red fluorescent staining 310 nm, 540 nm UV- and blue light
ROTI®Methylene blue staining concentrate 10 ng/band Reversible, non-toxic blue staining White light
ROTI®Black N <0.1 ng/band Silver staining of DNA polyacrylic amide gels White light

Certificates of Analysis

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