Technical Data Sheet
ROTI®Phenol/Chloroform/Isoamyl alcohol, 500 ml
Ready-to-use products and reagents
Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Boiling point (bp) >61 °C
Flash point (flp) >80 °C
Storage temp. +4 °C
ADR 6.1 III
WGK 3
UN-Nr. 2810
Covered with buffer. Store in the dark!
Redistilled, in TE-buffer equilibrated phenol, chloroform and isoamyl alcohol at a ratio of 25:24:1, pH 7,5-8,0.
ROTI®Phenol/Chloroform/Isoamyl alcohol has proven itself in many research laboratories for the isolation of nucleic acids and is an ideal complement to pure ROTI®Phenol.
€160.20/Pack Qty.
excl. VAT. | 500 ml per Pack Qty.
Art. No. A156.2
Fits to / Accessories
Product details
Please note our Technical Information Brochure on the Internet next to the products:
Phenolic DNA Isolation
Background and protocol
Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses.
For distinct phase separation, ROTI®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl.
- Reduce exposure to toxic chemicals
- Prepared from phenol of highest purity
- Packed under argon for maximum stability
- Successfully tried and tested in many research laboratories
ROTI®Phenol/Chloroform/Isoamyl alcohol ready-to-use, for the extraction of nucleic acids
ROTI®Phenol/Chloroform/Isoamyl alcohol is re-distilled phenol, equilibrated in TE buffer, premixed with high purity chloroform and isoamyl alcohol.
ROTI®Phenol/Chloroform/Isoamyl alcohol has proven itself in many research laboratories for the isolation of nucleic acids and is an ideal complement to pure ROTI®Phenol.
Addition of 1 volume ROTI®Phenol/Chloroform/Isoamyl alcohol and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase.
Draw solution from lower phase. Do not shake before use!
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| A156.1 | 250 ml | glass |
€94.50 |
|
|
| A156.2 | 500 ml | glass |
€160.20 |
|
|
| A156.3 | 100 ml | glass |
€47.20 |
|
|
|
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- Subtotal: 0.00
Downloads / MSDS
Certificates of Analysis
Notes on the product
| Bottled under argon. |