ROTI^®Plate90 VRBD

Sterile ready-to-use culture media plates for microbiological analysis.

ROTI^®Plate90 are prepared culture media plates with a diameter of 90 mm. They are thus adapted to the size of membrane filters for assaying of low-germ fluids by membrane filtration method. They are also applicable for the microbiological analysis of samples in general.

The germs of expected low-germ liquid samples are concentrated by membrane filtration. Afterwards, the membrane is placed on top of the medium and incubated. Using a membrane with a grid helps counting the resulting colonies.
Our products are tested and approved exclusively for research and development.

Samples which are expected to have a higher microbiological burden are prepared in a serial dilution. Afterwards, every dilution step is plated and incubated. The number of grown colonies is noted as result.

VRBD agar is used according to ISO 21528 to detect coliform bacteria as indicators of faecal contamination in water, food and feed.
Coliform bacteria ferment glucose and produce acid with or without gas. Lactose-negative Salmonella and Shigella species and enteropathogenic E. coli grow on this medium, as do Klebsiella and Citrobacter, which are more heat-resistant than coliforms and may indicate a production process defect (insufficient heating) of the products under investigation.
Gram-positive organisms are inhibited by the bile salts and crystal violet.