Technical Data Sheet
ROTI®Quick kit
Ready-to-use products and reagents
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Storage temp. +4 °C
ADR 3 II
WGK 3
UN-Nr. 2924
Kit for RNA-isolation from cells and tissues.
The ROTI®Quick Kit is suitable for use with cell culture material, biopsies, high-fat or high-protein tissue, blood (Üçeyler et al. Neurology (2007) 69:42-9), solid plant material, mitochondria (Backert et al. Plant Molecular Biology (1997) 33:1037-50), mycoplasms (Weiner et al. Nucleic Acids Res. (2000) 28:4488-96) and many more. The kit can be used for both fresh tissue and frozen material.
The ROTI®Quick Kit is based on the GITC isolation method by Chomczynski and Sacchi (Chomczynski and Sacchi. (1987) Anal. Biochem. 162:156) and includes three optimised ready-to-use solutions that were developed by molecular biologists and have proven effective for many years.
After the tissue has been homogenised, the RNA is purified in a single step and isolated by precipitation. Each isolation takes approximately 2,5 hours, and multiple isolations can easily be performed simultaneously. The incubation and centrifuging steps take about 2 hours.
€115.60/Pack Qty.
excl. VAT. | 1 kit per Pack Qty.
Art. No. A979.1
Fits to / Accessories
Product details
ROTI®Quick kit ready-to-use, for molecular biology
Column-free, ultra-flexible isolation system designed for reliable isolation of total RNA from almost any tissue.
The ROTI®Quick Kit is suitable for use with cell culture material, biopsies, high-fat or high-protein tissue, blood (Üçeyler et al. Neurology (2007) 69:42-9), solid plant material, mitochondria (Backert et al. Plant Molecular Biology (1997) 33:1037-50), mycoplasms (Weiner et al. Nucleic Acids Res. (2000) 28:4488-96) and many more. The kit can be used for both fresh tissue and frozen material.
The ROTI®Quick Kit is based on the GITC isolation method by Chomczynski and Sacchi (Chomczynski and Sacchi. (1987) Anal. Biochem. 162:156) and includes three optimised ready-to-use solutions that were developed by molecular biologists and have proven effective for many years.
After the tissue has been homogenised, the RNA is purified in a single step and isolated by precipitation. Each isolation takes approximately 2,5 hours, and multiple isolations can easily be performed simultaneously. The incubation and centrifuging steps take about 2 hours.
- Easy, most versatile application
- High recovery rate of intact, very pure total RNA
- Column-free two-step isolation
- Applicable on nearly any tissue type
Preparation as given in the instruction-for-use is optimised for RNA isolation from approx. 0,2 g of tissue or cell material. The amount of material isolated per batch may easily be up- or downscaled to larger or smaller amounts of tissue.
The isolated total RNA is highly pure and may be used directly for all common down-stream applications, such as RT-PCR, Northern blotting and reverse transcription. Detailed instructions-for-use are enclosed with each kit.
ROTI®Quick Kit 1, ROTI®Quick Kit 2 and ROTI®Quick Kit 3.
Contents of this Kit may not be bought separately.
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
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| A979.1 | 1 kit | cardboard | for approx. 20 preparations |
€115.60 |
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